predictprotein - blastall error

[giovani]

Hi,

I'm trying to run a predicprotein analysis locally,
but it stops at the first steps: converting blast output to saf
align.:

*** ERROR blastpgp_to_saf.pl : *** ERROR blastp_to_saf: blast file
format not recognized
Died at /usr/share/librg-utils-perl//blastpgp_to_saf.pl line 76.
make: ** [query.safBlastPsi80] Erro 255

I already run blast separatelly, and did the blast output to saf align
convergence,
with the same fasta sequence used before, and everything seems ok,

Does anybody faced similar issue or have any idea how to solve it?

Thanks.

[giovani]

Hi,

I tried a different database but the errors multiply,

this is the command line I used:
predictprotein --seqfile aminotransferase.fasta -o /
PredictProteinTestes/ --bigblastdb /BlastDatabases/nr.00/nr.00

and these are the output erros:

[blastpgp] WARNING: -t larger than 1 not supported when restarting
from a checkpoint; setting -t to 1
[blastpgp] WARNING: posReadCheckpoint: Attempting to recover data from
previous checkpoint
[blastpgp] WARNING: posReadPosFreqsStandard: Could not open checkpoint
file
[blastpgp] WARNING: posReadCheckpoint: Data recovery failed
[blastpgp] FATAL ERROR: blast: Error recovering from checkpoint
[blastall] FATAL ERROR: query: Database /mnt/project/rost_db/data/
blast/swiss was not found or does not exist

*** ERROR blastpgp_to_saf.pl : *** ERROR blastp_to_saf: blast file
format not recognized
Died at /usr/share/librg-utils-perl//blastpgp_to_saf.pl line 76.
make: ** [query.safBlastPsi80] Erro 255

make INFILE=query.in -C /tmp/Yfeym8BrlR JOBID=query -j 1 BLASTCORES=1
LIBRGUTILS=/usr/share/librg-utils-perl/ PPROOT=/usr/share/
predictprotein/ PROFNUMRESMIN=17 PROFROOT=/usr/share/profphd/prof/
PROFTMBROOT=/usr/share/proftmb/ BIGBLASTDB=/home/giovani/
BlastDatabases/nr.00/nr.00 BIG80BLASTDB=/mnt/project/rost_db/data/
blast/big_80 PFAM2DB=/mnt/project/rost_db/data/pfam/Pfam_ls PFAM3DB=/
mnt/project/rost_db/data/pfam/Pfam-A.hmm PROSITECONVDAT=/mnt/project/
rost_db/data/prosite/prosite_convert.dat PSICEXE=/usr/share/rost-
runpsic/runNewPSIC.pl SWISSBLASTDB=/mnt/project/rost_db/data/blast/
swiss --quiet -f /usr/share/predictprotein//MakefilePP.mk all failed:
512 at /usr/bin/predictprotein line 358.

Thanks