Opera senitivity, cell receptor numbers
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Dr. K. Koefoed
Nov 6, 2009, 7:28:21 AM11/6/09
to Opera High Content Imaging
Hi:
I was wondering if anyone in the Opera High Content Imaging group has
done experiments determining the sensitivity of the Opera system. In
particular I would like to know the limit of detection of cell
receptor / antigen numbers when employing primary and secondary
antibodies with or without wash steps.
If anyone has some experience on how to increase the sensitivity
(particular fluorochrome conjugates/polymers, TSA signal
amplification, plate types etc) I would also be very interested. I
have previously worked on the Applied Biosystems 8200 cellular
detection system. The limit of detection of this system is reported to
be 9000 receptors / cell in a homogenous format (Lee et al. J Biomol
Screen.2008; 13: 210-217). I would expect the sensitivity to be higher
on the Opera system but of cause comparisons are highly dependent on
the reagents and cells used. Just an estimate would be nice.
I was wondering if anyone in the Opera High Content Imaging group has
done experiments determining the sensitivity of the Opera system. In
particular I would like to know the limit of detection of cell
receptor / antigen numbers when employing primary and secondary
antibodies with or without wash steps.
If anyone has some experience on how to increase the sensitivity
(particular fluorochrome conjugates/polymers, TSA signal
amplification, plate types etc) I would also be very interested. I
have previously worked on the Applied Biosystems 8200 cellular
detection system. The limit of detection of this system is reported to
be 9000 receptors / cell in a homogenous format (Lee et al. J Biomol
Screen.2008; 13: 210-217). I would expect the sensitivity to be higher
on the Opera system but of cause comparisons are highly dependent on
the reagents and cells used. Just an estimate would be nice.
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