Ghislain 60 x bead images

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jringeling

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Jun 19, 2009, 10:50:32 AM6/19/09
to Opera High Content Imaging
Ok I just looked at the corrected bead images and I see the same kind
of issues I see on my TEHS

the beads in image 1 are 30-40 % brighter on the left side of the
image, which suggests an uneven image plane. Image 3 I assume is non
confocal? Obviously we don't even need to comment on image quality
there :)

How many people have seen this issue with intensity changes based on
where in the image the beads are located? Also, who else has a problem
with the fact that the SAT only uses the 75th percentile of the data?

Thanks
John
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