You do not have permission to delete messages in this group
Copy link
Report message
Show original message
Either email addresses are anonymous for this group or you need the view member email addresses permission to view the original message
to Opera High Content Imaging
Ok I just looked at the corrected bead images and I see the same kind
of issues I see on my TEHS
the beads in image 1 are 30-40 % brighter on the left side of the
image, which suggests an uneven image plane. Image 3 I assume is non
confocal? Obviously we don't even need to comment on image quality
there :)
How many people have seen this issue with intensity changes based on
where in the image the beads are located? Also, who else has a problem
with the fact that the SAT only uses the 75th percentile of the data?