Downstream effects of carrier RNA in RAD/ddRAD

[Jenny Kaden]

Hello all,

Bit of a wet lab question. 

Does anyone know the effect carrier RNA might have on a ddRAD library?

We've done a couple of ddRAD libraries but I'm currently trying to extract enough DNA from some old feathers to run the next one. We're getting a much better yield of DNA using a QIAamp Investigator Kit (with carrier RNA), but we're not sure if we can leave this in for the ddRAD library, or if we will have to perform an RNase clean up before using the samples (which would take a lot of time).

Thanks very much in advance for any suggestions/thoughts you might have.

Jenny