Hello, everyone
I have a question about peakBin()
Here is a spectra of initial data:
![Screenshot 2023-03-15 at 18.44.32.png](https://groups.google.com/group/CardinalMSI/attach/b62365219d8c9/Screenshot%202023-03-15%20at%2018.44.32.png?part=0.1&view=1)
After such steps as
peakPick()
peakAlign()
peakFilter()
I applied
file_P_A_F_B <- file_P_A_F%>%
peakBin(ref = mz(file_P_A_F), tolerance=10, units="ppm")%>%
process()
and a lot of peaks disappeared:
![b9891e29-7678-45d1-ad44-91b792e80fc4.png](https://groups.google.com/group/CardinalMSI/attach/b62365219d8c9/b9891e29-7678-45d1-ad44-91b792e80fc4.png?part=0.2&view=1)
however, if I take bigger tolerance, it duplicates peaks:
this pictures are all for one pixel
please could you explain how use peakBin correctly?
thank in advance
Marina