peakBin

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Marina Zavolskova

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Mar 15, 2023, 7:59:21 AM3/15/23
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Hello, everyone
I have a question about peakBin()
Here is a spectra of initial data:Screenshot 2023-03-15 at 18.44.32.png
After such steps as 
peakPick()
peakAlign()
peakFilter()
I applied 
file_P_A_F_B <- file_P_A_F%>%
     peakBin(ref = mz(file_P_A_F), tolerance=10, units="ppm")%>%
     process()
and a lot of peaks disappeared:b9891e29-7678-45d1-ad44-91b792e80fc4.png
however, if I take bigger tolerance, it duplicates peaks:
8577744b-17ec-4a92-8a72-0d916fc962b9.png
this pictures are all for one pixel
please could you explain how use peakBin correctly?
thank in advance
Marina

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