After manual correction of juicebox convert to fasta

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abdullah padana

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Sep 7, 2023, 7:27:51 AM9/7/23
to 3D Genomics
Hi Juicebox team,

java -Xmx32G -jar juicer_tools.2.20.00.jar post -o output abdullah_manual_out_JBAT.review.assembly out_JBAT.liftover.agp yahs.out_scaffolds_final.fa

WARNING: sun.reflect.Reflection.getCallerClass is not supported. This will impact performance.
WARN [2023-09-07T20:51:58,793]  [Globals.java:138] [main]  Development mode is enabled
Exception in thread "main" jargs.gnu.CmdLineParser$UnknownOptionException: Unknown option '-o'
at jargs.gnu.CmdLineParser.parse(CmdLineParser.java:475)
at jargs.gnu.CmdLineParser.parse(CmdLineParser.java:426)
at juicebox.tools.HiCTools.main(HiCTools.java:67)

please can you guide me about this error. Thanks 

Olga Dudchenko

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Sep 8, 2023, 3:37:51 PM9/8/23
to 3D Genomics
You are not using the right call. The correct call for JBAT/3D-DNA suite is the 3d-dna script for post review. Depending on the release you are using it is ether a standalone post-review script or the -r option in the main run-3ddna-assembler script. See Genome Assembly Cookbook (dnazoo.org/methods) for more details instructions. -Olga 
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