Error: the chromosome combination 1_2 appears in multiple blocks

[Yuying Ding]

Hi everyonre i'm using jucer tools to generate hic file, when i run this code

java -Xmx48g -jar juicer_tools_1.22.01.jar pre -j 48 sorted_chm13_filtered_A562-pcc_interaction_df.txt A562.hicchm13.chrom.sizes

and i got the error below:

WARNING: sun.reflect.Reflection.getCallerClass is not supported. This will impact performance.
WARN [2024-10-11T11:26:11,814]  [Globals.java:138] [main]  Development mode is enabled
Using 48 CPU thread(s)
Not including fragment map
Start preprocess
Writing header
Writing body
.........................Error: the chromosome combination 1_2 appears in multiple blocks

i've read a lot same error before and checked my file by:

awk '{if ($3 == "chr1" && $7 == "chr2") {print NR,$0; exit}}' sorted_chm13_filtered_A562-pcc_interaction_df.txt

with the result:

3707831 00006b37-441c-47dd-916d-b8a980e714b0 -1 chr1 36417280 36416721 0 chr2 17550468 17550193 51 51
awk '{if ($3 == "chr2" && $7 == "chr1") {print NR,$0; exit}}' sorted_chm13_filtered_A562-pcc_interaction_df.txt
**nothing return***

I would appreciate any help with this error. Thank you in advance!

[Yuying Ding]

ohhhh i thought i've found whats wrong. i must sort fragments by the position! 
now everything looks right!