Correct format for Hi-C matrix before converting to BEDPE

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despoina tsa

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Mar 2, 2025, 6:23:43 PM3/2/25
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I am working with interchromosomal Hi-C interaction data and need to extract a matrix from a .hic file using Juicer Tools (v2.13.06). My goal is to convert this matrix into BEDPE format for downstream analysis.

Currently, when I use the following command:

java -jar juicer_tools_2.13.06.jar dump observed NONE contact_matrix_GRCh38.hic 1 2 BP 1000000 chr1_chr2.matrix

i get an output like this :

0    0    13.0  
1000000    0    79.0  
2000000    0    99.0  
3000000    0    153.0  


So i have the following questions if anyone can answer it would be very helpful : 1)What is the correct matrix format before converting to BEDPE? 2)Should the output include chromosome names, or do I need to add them manually? 3)Are there any additional steps I should take to format the data correctly for BEDPE conversion?


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