I am working with interchromosomal Hi-C interaction data and need to extract a matrix from a .hic file using Juicer Tools (v2.13.06). My goal is to convert this matrix into BEDPE format for downstream analysis.
Currently, when I use the following command:
java -jar juicer_tools_2.13.06.jar dump observed NONE contact_matrix_GRCh38.hic 1 2 BP 1000000 chr1_chr2.matrixi get an output like this :
0 0 13.0
1000000 0 79.0
2000000 0 99.0
3000000 0 153.0
So i have the following questions if anyone can answer it would be very helpful : 1)What is the correct matrix format before converting to BEDPE? 2)Should the output include chromosome names, or do I need to add them manually? 3)Are there any additional steps I should take to format the data correctly for BEDPE conversion?