APA error

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kate

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Sep 5, 2021, 4:08:36 AM9/5/21
to 3D Genomics
Hello,
I am trying APA with the latest jar (1.22.01) on SLURM.
However, I got the following err:

Processing APA for resolution 20000

Exception in thread "main" java.lang.IllegalArgumentException

at java.util.concurrent.ThreadPoolExecutor.<init>(ThreadPoolExecutor.java:1307)

at java.util.concurrent.ThreadPoolExecutor.<init>(ThreadPoolExecutor.java:1195)

at java.util.concurrent.Executors.newFixedThreadPool(Executors.java:89)

at juicebox.tools.clt.juicer.APA.runWithReturn(APA.java:298)

at juicebox.tools.clt.juicer.APA.run(APA.java:223)

at juicebox.tools.HiCTools.main(HiCTools.java:94)

Would you please help me figure out the problem? I'm using java/jdk-11.0.11 and  cuda/8.0.
Thank you,
Kate

Neva Durand

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Sep 5, 2021, 9:28:43 AM9/5/21
to kate, 3D Genomics
What is your command?

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Muhammad Saad Shamim

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Sep 5, 2021, 1:39:55 PM9/5/21
to Neva Durand, kate, 3D Genomics
You may want to use --threads 1 or an older jar. 

Kate

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Sep 5, 2021, 1:57:33 PM9/5/21
to Muhammad Saad Shamim, Neva Durand, 3D Genomics
Hello,
--threads1 is working! Can I ask you one more question? 
I want to use CenterNormedAPA and APA to compare the strength of looping interaction at the specific loop anchors between control and treated sample. 
To do this, I export the values to R and draw heat map with the same scale (color)-range for two samples.
But I'm not sure if I really need to adjust the scale-range to the same for comparison. Do I need to do that or P2LL score tells everything (significance >1)? The original png files show all different color scales. 

Thank you!
Kate

2021년 9월 5일 (일) 오후 1:39, Muhammad Saad Shamim <sa50...@gmail.com>님이 작성:

Ashley S Doane

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Oct 6, 2021, 3:21:10 PM10/6/21
to 3D Genomics
Thanks I had the same error and this solved it.   Is this a java version issue?  Would be great to add this to documentation somewhere as it took awhile to track down solution.
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