Re: Not including fragment map?

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Neva Durand

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Mar 22, 2018, 8:27:08 AM3/22/18
to shen Eric, 3D Genomics
Look at your other log files. For example for launching the jobs it should tell you what site file. You might have specified a file path that it couldn’t find when it ran. 

What was the command you used to run?

On Thu, Mar 22, 2018 at 7:57 AM shen Eric <swei...@gmail.com> wrote:
Hi,

    I just found that my result hic file doesn't contain fragment resolusioin. and I checked the shell script and log file.

    I did specify a site_file, but why the juicebox_tools pre logfile says "Not including fragment map"?

Thanks

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Neva Cherniavsky Durand, Ph.D.
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Neva Durand

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Mar 27, 2018, 9:33:56 AM3/27/18
to shen Eric, 3D Genomics
Does your restriction site file exist at /path/to/dm3_DpnII.txt  ?

On Fri, Mar 23, 2018 at 10:06 PM, shen Eric <swei...@gmail.com> wrote:

Here is my command :
juicer.sh -g dm3 -s DpnII -p  /path/to/dm3.chrom.sizes -y /path/to/dm3_DpnII.txt -z /path/to/dm3.fa


在 2018年3月22日星期四 UTC+8下午8:27:08,Neva Durand写道:
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Neva Durand

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Apr 8, 2018, 7:49:13 AM4/8/18
to shen Eric, 3D Genomics
What was your command using Juicer Tools pre?

On Sun, Apr 8, 2018 at 4:22 AM, shen Eric <swei...@gmail.com> wrote:
sorry for the delay, but I checked these path and I'm sure they are correct.
And I tried to use juicerbox_tools.jar pre to generate .hic file, still didn't get fragment resolusion.

在 2018年3月27日星期二 UTC+8下午9:33:56,Neva Durand写道:
Does your restriction site file exist at /path/to/dm3_DpnII.txt  ?
On Fri, Mar 23, 2018 at 10:06 PM, shen Eric <swei...@gmail.com> wrote:

Here is my command :
juicer.sh -g dm3 -s DpnII -p  /path/to/dm3.chrom.sizes -y /path/to/dm3_DpnII.txt -z /path/to/dm3.fa


在 2018年3月22日星期四 UTC+8下午8:27:08,Neva Durand写道:
Look at your other log files. For example for launching the jobs it should tell you what site file. You might have specified a file path that it couldn’t find when it ran. 

What was the command you used to run?

On Thu, Mar 22, 2018 at 7:57 AM shen Eric <swei...@gmail.com> wrote:
Hi,

    I just found that my result hic file doesn't contain fragment resolusioin. and I checked the shell script and log file.

    I did specify a site_file, but why the juicebox_tools pre logfile says "Not including fragment map"?

Thanks

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Neva Cherniavsky Durand, Ph.D.
Staff Scientist, Aiden Lab

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Neva Cherniavsky Durand, Ph.D.
Staff Scientist, Aiden Lab

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shen Eric

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Apr 22, 2018, 10:50:36 AM4/22/18
to 3D Genomics
I found the reason.
It's because I use an old version of juicer so there is a wrong if [] in juicer.sh when runing juicebox_tools pre job, so it will always run without fragment resolusion.

在 2018年4月8日星期日 UTC+8下午7:49:13,Neva Durand写道:

feng...@gmail.com

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Apr 23, 2018, 6:27:23 AM4/23/18
to 3D Genomics
I also get the same report Not including fragment map,so could you tell me which line of the code cause the problem?thank you .

在 2018年4月22日星期日 UTC+8下午10:50:36,shen Eric写道:

Neva Durand

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Apr 23, 2018, 2:01:46 PM4/23/18
to feng...@gmail.com, 3D Genomics
Check the help message when you run Juicer. We now by default do not include fragment maps in the HiC file but you can turn it on. 


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shen Eric

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Apr 26, 2018, 3:02:40 AM4/26/18
to 3D Genomics

You can update your juicer.sh script, this may solve the problem.


在 2018年4月23日星期一 UTC+8下午6:27:23,feng...@gmail.com写道:
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