|Best way to clean cuvettes?||Matthew Parker||10/15/96 12:00 AM|
Hi, folks! Does anybody have any suggestions about a good way to clean
proteins out of quartz cuvettes? I have used concentrated nitric acid in
the past, and also 3M HCl/50% ethanol (which doesn't seem to work as
well, at least in my experience). Is there anything else that works well
(say, 50% nitric)?
|Best way to clean cuvettes?||Cornelius Krasel||10/16/96 12:00 AM|
A mixture of potassium chromate and 100% sulfuric acid is the traditional
|Best way to clean cuvettes?||Jesus Sanz||10/16/96 12:00 AM|
In article <3263EF...@topaz.microbio.uab.edu>,
I use Hellmanex II solution (from Helma) at 2% for at least
|Best way to clean cuvettes?||Achim Recktenwald, PhD||10/16/96 12:00 AM|
|Best way to clean cuvettes?||Joe Hilario||10/16/96 12:00 AM|
try soaking your cuvettes for a few hours in Aqua Regia (3:1 HCl:HNO3)
|Best way to clean cuvettes?||Chartchai Krittanai||10/16/96 12:00 AM|
In our lab, we fill the cuvette with 2% of RBS(from Pierce,
Hope this helps.
|Best way to clean cuvettes?||Pete||10/23/96 12:00 AM|
>Matthew Parker (par...@topaz.microbio.uab.edu) wrote:
We just use the high UV transmission disposable methacrylate cuvettes
|Best way to clean cuvettes?||z||10/24/96 12:00 AM|
In artikeltje <54kr3l$8...@info-server.surrey.ac.uk>, Wed, 23 Oct 1996 10:39:21 GMT,
aren't these expensive ? quartz cuvettes are best cleaned with a warm (60C)
|Best way to clean cuvettes?||todd andrew johnson||10/25/96 12:00 AM|
How often does your lab clean your cuvettes after each sample, set
|Best way to clean cuvettes?||Matthew Parker||10/26/96 12:00 AM|
I'm talking about between runs; we do kinetic assays in the cuvettes,
and trace amounts left over from the previous assay can mess things up.
|Best way to clean cuvettes?||Sandra Graham-Lorence||10/28/96 12:00 AM|
Well, we also have to clean them between each run, as the enzyme is
very sensitive to the presence of substrate, and the substrate sticks
everywhere (fatty acids). Personnally I wash the cuvette with water and
soap (regular detergent) and a cottonswab. I rinse a lot (10 times or
more) with deionised water and then rinse about 10 times with methanol.
Like that I don't have to do an extra wash every so often, it is always
clean (although a little bit time consuming, but results are clean,
|Best way to clean cuvettes?||Joe Hilario||10/28/96 12:00 AM|
if you clean cuvettes between runs..
typically we clean the cuvettes with 95% ethanol thoroughly and then with
Joe Hilario In a sea of faces, in a sea of doubt
|Best way to clean cuvettes?||Achim Recktenwald, PhD||10/30/96 12:00 AM|
|Best way to clean cuvettes?||Pete||11/1/96 12:00 AM|
un6...@genius.embnet.dkfz-heidelberg.de (z) wrote:
<snipped for brevity>
>aren't these expensive ? quartz cuvettes are best cleaned with a warm (60C)
I agree with your comments on cleaning, although we use 2% Decon.
But regarding cost of disposable cuvettes, I do not think that they
|Best way to clean cuvettes?||clkst...@postoffice.worldnet.att.net||11/5/96 12:00 AM|
I have used both quartz and disposable, and quatrz bears the bell away as
far as reproducibility. Call me old-fashioned, but I would trust a result
from a quartz cuvette ove a result from a plastic one anyday.
|Best way to clean cuvettes?||Achim Recktenwald, PhD||11/7/96 12:00 AM|