Genome Engineering using CRISPR/Cas Systems

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New to CRISPR/Cas9 for gene knockout -- Need help with PX458 and PX459 v2.0 nsim...@gmail.com 7:58 AM
pSpCas9(BB)-2A-Puro (PX459) V2.0 (Plasmid #62988) cloning methods Sean Collier 7:39 AM
Puromycin selection for pSPCas9(BB)-2A-Puro v2.0 Neuropath 7:14 AM
Synthego synthetic sgRNA for CRISPR - FOR FREE (3 targets!) kevin....@synthego.com 5:56 AM
5 Free Passes Available for the Genome Editing UK Congress **EXCLUSIVE LIMITED OFFER** Danielle Meredith 3:33 AM
NgAgo Davide Seruggia 3:11 AM
Advise about HA length for GFP knock-in reporter in uman ES Dario 12:36 AM
The problem of low efficiency about electroporation transformation daqi wang 9/28/16
CRISPR efficiency/ success rate regulatingge...@gmail.com 9/28/16
Use of lentiCRISPRv2 for THP1 transfection ei...@thsti.res.in 9/28/16
error in running guide-seq govin 9/27/16
Is it possible to use CRISPR/Cas9 system on bacteria that already have Cas9 in their genome? Pietro Fantocci 9/27/16
Sequencing primers for the first PCR of GeCKO library amplification luigia...@gmail.com 9/27/16
lentiCpf1 Aaron 9/27/16
Inducible Cas9 + transfected sgRNA Gopal J 9/27/16
Failure to clone guides into vector imaca...@gmail.com 9/26/16
Inducible Cas9 and inducible gRNA in the same cell line les...@gmail.com 9/26/16
New preprint: multiplex tRNA-gRNA system and Cpf1 Fil 9/25/16
Positive colonies-failed sequencing nikita modi 9/25/16
Donor plasmid homology arms in an inverted orientation Miguel Vizoso 9/23/16
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