Genome Engineering using CRISPR/Cas Systems

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gibson SAM protocol Aaron 2:14 AM
lentiCRISPRv2 used in human iPS Stem Cells? Aaron Tang 6/24/16
Dose anyone has protocol for transfection and puro selection? Jayci Huang 6/24/16
CRISPResso: Analysis of CRISPR-Cas9 genome editing outcomes from deep sequencing data NOW ONLINE! Luca Pinello 6/24/16
NgAgo Davide Seruggia 6/24/16
sgRNA for RNP nucleofection: order custom RNA, or in vitro transcription? 6/24/16
problems in insert cloning in pX459 Constanza Espada 6/24/16
missing sgRNAs in GeCKO library Elizabeth S 6/24/16
RNA oligo from IDT as gRNA for Cpf1? Paddy 6/24/16
REMINDER: FREE CRISPR webinar on June 27 at 12pm Eugenia Petrova 6/24/16
Fluorescent Tagging ssODN for HDR repair template Josh 6/24/16
SCR7 PDAC-UCLA 6/24/16
Prokaryotic terminators for gRNA vector expression Hannah D 6/24/16
Re: Point mutation making a PAM sequence 6/23/16
Order human KO cell lines on DESKGEN! Eugenia Petrova 6/23/16
Cpf1 for generating knock-in lines? Dani Swain 6/22/16
Lentiviral sgRNA vector with Neomycin resistance Davide Seruggia 6/22/16
annealing of oligos Hussein Chhouri 6/22/16
RE: DNA-guided genome editing using the Natronobacterium gregoryi Argonaute SKim 6/21/16
2gRNA + Cas9 + ds oligo - to achieve point mutation via HDR Viral Shah 6/21/16
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