Across sample normalization using ERCC spike-ins on single-cell RNA-seq datasets after Salmon quantification

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Gary Ho

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Dec 1, 2016, 4:16:11 AM12/1/16

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Hi all,

Does anybody know how to normalize salmon quantification output using ERCC spike-ins? I would like to use Salmon to analyze my single-cell RNA-seq data on transcript-level.

Appreciate any suggestion or comment~

Best!

Gary

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