command line to estimate only the best-scoring tree

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Juan C. Opazo

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Dec 8, 2015, 2:17:22 PM12/8/15
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Hi guys, I am wondering if you can help me setting up the command line to only obtain the best-scoring ML tree (no bootstrap).  I tried:

raxmlHPC-MPI -f d -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n outfile -# 20

but I am getting a collection of 20 trees, but not the RAxML.bestTree file.

any help is welcome

Juan

Alexandros Stamatakis

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Dec 8, 2015, 4:29:29 PM12/8/15
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hi juan,

that's weird, which version of RAxML are you using?

alexis
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Alexandros (Alexis) Stamatakis

Research Group Leader, Heidelberg Institute for Theoretical Studies
Full Professor, Dept. of Informatics, Karlsruhe Institute of Technology
Adjunct Professor, Dept. of Ecology and Evolutionary Biology, University
of Arizona at Tucson

www.exelixis-lab.org

Juan C. Opazo

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Dec 8, 2015, 4:47:42 PM12/8/15
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Hi Alexis, thank you for your rapid response, I am using RAxML version 8.2.0. I am wondering if is possible to only obtain the best tree, no bootstrap. The reason is that most of the times before to proceed to the bootstrap analysis on the final data set, we explore our data using best trees (because obtaining them is very fast). So in our lab we make tons of best trees before to close our data set and perform support routines (e.g. bootstrap and Bayesian analyses).

Now I tried the following command line which also perform bootstrap, but my trick is to set the -# setting to 2,

raxmlHPC-MPI -f a -p 12345 -s Alignment.fasta -x 12345 -# 2 -m PROTGAMMAJTT -n outfile

any help is welcome

juan




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--
Juan C. Opazo
Instituto de Cs. Ambientales y Evolutivas
Facultad de Ciencias
Universidad Austral de Chile
Casilla 567, Valdivia
Chile
fono: 56-63-2221674
fax: 56-63-2221344
jopazo (at) gmail (dot) com

OpazoLab
ResearchGate
Doctorado en Ciencias mención Ecología y Evolución

Alexandros Stamatakis

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Dec 8, 2015, 4:59:28 PM12/8/15
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Hi Juan,

Could it be that your batch queueing system is recognizing -# 20 as
comment, maybe you should try -N 20 which is equivalent.

Also please upgrade to the latest version 8.2.4

Finally, I looked at the code and it should produce a best-scoring tree
file, the end of the output should look something like this below.

Alexis

Starting final GAMMA-based thorough Optimization on tree 11 likelihood
-116.492312 ....

Final GAMMA-based Score of best tree -116.492312

Program execution info written to
/home/stamatak/Desktop/GIT/raxml-hpc/standard-RAxML/RAxML_info.PP6
Best-scoring ML tree written to:
/home/stamatak/Desktop/GIT/raxml-hpc/standard-RAxML/RAxML_bestTree.PP6

Overall execution time: 1.614655 secs or 0.000449 hours or 0.000019 days




On 08.12.2015 22:47, Juan C. Opazo wrote:
> Hi Alexis, thank you for your rapid response, I am using RAxML version
> 8.2.0. I am wondering if is possible to only obtain the best tree, no
> bootstrap. The reason is that most of the times before to proceed to the
> bootstrap analysis on the final data set, we explore our data using best
> trees (because obtaining them is very fast). So in our lab we make tons
> of best trees before to close our data set and perform support routines
> (e.g. bootstrap and Bayesian analyses).
>
> Now I tried the following command line which also perform bootstrap, but
> my trick is to set the -# setting to 2,
>
> raxmlHPC-MPI -f a -p 12345 -s Alignment.fasta -x 12345 -# 2 -m
> PROTGAMMAJTT -n outfile
>
> any help is welcome
>
> juan
>
>
>
>
> On Tue, Dec 8, 2015 at 6:29 PM, Alexandros Stamatakis
> <alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>> wrote:
>
> hi juan,
>
> that's weird, which version of RAxML are you using?
>
> alexis
>
> On 08.12.2015 20:17, Juan C. Opazo wrote:
>
> Hi guys, I am wondering if you can help me setting up the
> command line
> to only obtain the best-scoring ML tree (no bootstrap). I tried:
>
> raxmlHPC-MPI -f d -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n
> outfile
> -# 20
>
> but I am getting a collection of 20 trees, but not the
> RAxML.bestTree file.
>
> any help is welcome
>
> Juan
>
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>
>
> --
> Alexandros (Alexis) Stamatakis
>
> Research Group Leader, Heidelberg Institute for Theoretical Studies
> Full Professor, Dept. of Informatics, Karlsruhe Institute of Technology
> Adjunct Professor, Dept. of Ecology and Evolutionary Biology, University
> of Arizona at Tucson
>
> www.exelixis-lab.org <http://www.exelixis-lab.org>
>
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>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674
> fax: 56-63-2221344
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención Ecología y Evolución <http://www.ecolevol.cl>

Juan C. Opazo

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Dec 8, 2015, 5:02:56 PM12/8/15
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Thank you Alexis, I´ll check this out and I´ll upgrade to the recommended version.

best

juan

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--
Juan C. Opazo
Instituto de Cs. Ambientales y Evolutivas
Facultad de Ciencias
Universidad Austral de Chile
Casilla 567, Valdivia
Chile
fono: 56-63-2221674
fax: 56-63-2221344
jopazo (at) gmail (dot) com

OpazoLab

Juan C. Opazo

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Dec 8, 2015, 6:44:58 PM12/8/15
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Hi Alexis, no luck....I changed -# by -N as you suggested but nothing.......here is the output

do you have any idea?

best

juan

cbuach@ubuntu-frontend:~/Desktop/prueba_RAxML$ raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n outfile -N 10

This is RAxML MPI Process Number: 0

RAxML can't, parse the alignment file as phylip file
it will now try to parse it as FASTA file



This is RAxML version 8.2.0 released by Alexandros Stamatakis on July 9 2015.

With greatly appreciated code contributions by:
Andre Aberer      (HITS)
Simon Berger      (HITS)
Alexey Kozlov     (HITS)
Kassian Kobert    (HITS)
David Dao         (KIT and HITS)
Nick Pattengale   (Sandia)
Wayne Pfeiffer    (SDSC)
Akifumi S. Tanabe (NRIFS)

Alignment has 363 distinct alignment patterns

Proportion of gaps and completely undetermined characters in this alignment: 1.62%

RAxML rapid hill-climbing mode

Using 1 distinct models/data partitions with joint branch length optimization


Executing 10 inferences on the original alignment using 10 distinct randomized MP trees

All free model parameters will be estimated by RAxML
GAMMA model of rate heteorgeneity, ML estimate of alpha-parameter

GAMMA Model parameters will be estimated up to an accuracy of 0.1000000000 Log Likelihood units

Partition: 0
Alignment Patterns: 363
Name: No Name Provided
DataType: AA
Substitution Matrix: JTT
Using fixed base frequencies




RAxML was called as follows:

raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n outfile -N 10


Partition: 0 with name: No Name Provided
Base frequencies: 0.077 0.052 0.043 0.052 0.020 0.041 0.062 0.073 0.023 0.054 0.092 0.059 0.024 0.040 0.051 0.069 0.059 0.014 0.032 0.066

Inference[0]: Time 23.651726 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[1]: Time 23.689768 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[2]: Time 23.367036 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[3]: Time 23.416103 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[4]: Time 23.372482 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[5]: Time 23.381697 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[6]: Time 23.303497 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[7]: Time 23.453993 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[8]: Time 23.775530 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[9]: Time 23.782873 GAMMA-based likelihood -6306.468434, best rearrangement setting 5


Conducting final model optimizations on all 10 trees under GAMMA-based models ....

Inference[0] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.0
Inference[1] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.1
Inference[2] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.2
Inference[3] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.3
Inference[4] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.4
Inference[5] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.5
Inference[6] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.6
Inference[7] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.7
Inference[8] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.8
Inference[9] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.9
cbuach@ubuntu-frontend:~/Desktop/prueba_RAxML$

Alexey Kozlov

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Dec 8, 2015, 7:19:26 PM12/8/15
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Dear Juan,

could you try it with more than 1 MPI process? Alternatively, just use sequential version of RAxML if you don't need
parallelization. This should fix the problem.

@Alexis: bestProcess is only initialized if processes > 1, looks like a bug.

Alexey
> <mailto:alexandros...@gmail.com <mailto:alexandros...@gmail.com>>> wrote:
>
> hi juan,
>
> that's weird, which version of RAxML are you using?
>
> alexis
>
> On 08.12.2015 20:17, Juan C. Opazo wrote:
>
> Hi guys, I am wondering if you can help me setting up the
> command line
> to only obtain the best-scoring ML tree (no bootstrap). I tried:
>
> raxmlHPC-MPI -f d -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n
> outfile
> -# 20
>
> but I am getting a collection of 20 trees, but not the
> RAxML.bestTree file.
>
> any help is welcome
>
> Juan
>
> --
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> For more options, visit https://groups.google.com/d/optout.
>
>
> --
> Alexandros (Alexis) Stamatakis
>
> Research Group Leader, Heidelberg Institute for Theoretical Studies
> Full Professor, Dept. of Informatics, Karlsruhe Institute of Technology
> Adjunct Professor, Dept. of Ecology and Evolutionary Biology, University
> of Arizona at Tucson
>
> www.exelixis-lab.org <http://www.exelixis-lab.org> <http://www.exelixis-lab.org>
>
> --
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> For more options, visit https://groups.google.com/d/optout.
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674 <tel:56-63-2221674>
> fax: 56-63-2221344 <tel:56-63-2221344>
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención Ecología y Evolución <http://www.ecolevol.cl>
>
> --
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>
>
> --
> Alexandros (Alexis) Stamatakis
>
> Research Group Leader, Heidelberg Institute for Theoretical Studies
> Full Professor, Dept. of Informatics, Karlsruhe Institute of Technology
> Adjunct Professor, Dept. of Ecology and Evolutionary Biology, University
> of Arizona at Tucson
>
> www.exelixis-lab.org <http://www.exelixis-lab.org>
>
> --
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>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674 <tel:56-63-2221674>
> fax: 56-63-2221344 <tel:56-63-2221344>

Juan C. Opazo

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Dec 8, 2015, 7:32:27 PM12/8/15
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Hi Alex, do you mean adding a -T 2 for example? so the command would be:

cbuach@ubuntu-frontend:~/Desktop/prueba_RAxML$ raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n outfile -N 10 -T 2

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--
Juan C. Opazo
Instituto de Cs. Ambientales y Evolutivas
Facultad de Ciencias
Universidad Austral de Chile
Casilla 567, Valdivia
Chile
fono: 56-63-2221674
fax: 56-63-2221344
jopazo (at) gmail (dot) com

OpazoLab

Alexey Kozlov

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Dec 8, 2015, 7:36:32 PM12/8/15
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No, -T parameter is for PTHREADS version only (which you could also try BTW), but if you want to use MPI you'd need
something like:

cbuach@ubuntu-frontend:~/Desktop/prueba_RAxML$ mpirun -n 2 raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n
outfile

Alexey

On 09.12.2015 01:32, Juan C. Opazo wrote:
> Hi Alex, do you mean adding a -T 2 for example? so the command would be:
>
> cbuach@ubuntu-frontend:~/Desktop/prueba_RAxML$ raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n outfile -N 10
> -T 2
>
> <mailto:raxml%2Bunsu...@googlegroups.com
> <mailto:raxml%252Buns...@googlegroups.com> <mailto:raxml%252Buns...@googlegroups.com
> <mailto:raxml%25252Bun...@googlegroups.com>>>
> <mailto:raxml%252Buns...@googlegroups.com> <mailto:raxml%252Buns...@googlegroups.com
> <mailto:raxml%25252Bun...@googlegroups.com>>>>.
> For more options, visit https://groups.google.com/d/optout.
>
>
> --
> Alexandros (Alexis) Stamatakis
>
> Research Group Leader, Heidelberg Institute for Theoretical Studies
> Full Professor, Dept. of Informatics, Karlsruhe Institute of Technology
> Adjunct Professor, Dept. of Ecology and Evolutionary Biology, University
> of Arizona at Tucson
>
> www.exelixis-lab.org <http://www.exelixis-lab.org> <http://www.exelixis-lab.org> <http://www.exelixis-lab.org>
>
> --
> You received this message because you are subscribed to a topic in
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> For more options, visit https://groups.google.com/d/optout.
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674 <tel:56-63-2221674> <tel:56-63-2221674 <tel:56-63-2221674>>
> fax: 56-63-2221344 <tel:56-63-2221344> <tel:56-63-2221344 <tel:56-63-2221344>>
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención Ecología y Evolución <http://www.ecolevol.cl>
>
> --
> You received this message because you are subscribed to the Google
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> To unsubscribe from this group and stop receiving emails from it, send
> an email to raxml+un...@googlegroups.com <mailto:raxml%2Bunsu...@googlegroups.com>
> fono: 56-63-2221674 <tel:56-63-2221674> <tel:56-63-2221674 <tel:56-63-2221674>>
> fax: 56-63-2221344 <tel:56-63-2221344> <tel:56-63-2221344 <tel:56-63-2221344>>
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención Ecología y Evolución <http://www.ecolevol.cl>
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674 <tel:56-63-2221674>
> fax: 56-63-2221344 <tel:56-63-2221344>
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención Ecología y Evolución <http://www.ecolevol.cl>
>
> --
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>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674

Juan C. Opazo

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Dec 8, 2015, 7:45:52 PM12/8/15
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Bingo! now it works!

thank you very much you all your time!

all the best

juan

here is the output, just in case

cbuach@ubuntu-frontend:~/Desktop/prueba_RAxML$ mpirun -n 2 raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAJTT -n outfile -N 10


This is RAxML MPI Process Number: 0

This is RAxML MPI Process Number: 1
Inference[5]: Time 23.634978 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[0]: Time 23.636933 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[6]: Time 23.568601 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[1]: Time 23.700877 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[7]: Time 23.467421 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[2]: Time 23.379018 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[3]: Time 23.410665 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[8]: Time 23.633969 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[4]: Time 23.364898 GAMMA-based likelihood -6306.468434, best rearrangement setting 5
Inference[9]: Time 23.639813 GAMMA-based likelihood -6306.468434, best rearrangement setting 5



Conducting final model optimizations on all 10 trees under GAMMA-based models ....

Inference[5] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.5
Inference[0] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.0
Inference[1] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.1
Inference[6] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.6
Inference[2] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.2
Inference[7] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.7
Inference[3] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.3
Inference[8] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.8
Inference[4] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.4
Inference[9] final GAMMA-based Likelihood: -6306.468434 tree written to file /home/cbuach/Desktop/prueba_RAxML/RAxML_result.outfile.RUN.9


Starting final GAMMA-based thorough Optimization on tree 9 likelihood -6306.468434 ....

Final GAMMA-based Score of best tree -6306.468434

Program execution info written to /home/cbuach/Desktop/prueba_RAxML/RAxML_info.outfile
Best-scoring ML tree written to: /home/cbuach/Desktop/prueba_RAxML/RAxML_bestTree.outfile

Overall execution time: 126.315319 secs or 0.035088 hours or 0.001462 days

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Juan C. Opazo
Instituto de Cs. Ambientales y Evolutivas
Facultad de Ciencias
Universidad Austral de Chile
Casilla 567, Valdivia
Chile
fono: 56-63-2221674
fax: 56-63-2221344
jopazo (at) gmail (dot) com

OpazoLab

Juan C. Opazo

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Dec 8, 2015, 8:08:31 PM12/8/15
to raxml
Hi Alex, one more question I am also defining the command to select the best model for amino acid alignments. At the beginning I used:

raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAAUTO --auto-prot=aicc -n outfile -# 2

and I got the model, nothing else.

After including the "mpirun -n 2"as you suggested:

mpirun -n 2 raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAAUTO --auto-prot=aicc -n outfile -# 2

I am also obtaining the best tree. Is that ok? the best tree reconstruction is using the best model selected?

best

juan
 

Alexandros Stamatakis

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Dec 9, 2015, 4:56:06 AM12/9/15
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Hi Juan,

Note that Alexey also answered on here (he is my PhD student) so it was
not only me who helped, I admit the names are pretty similar.

Alexey, I've noted that I need to include a check for #processes < 2

> raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAAUTO
> --auto-prot=aicc -n outfile -# 2
>
> and I got the model, nothing else.
>
> After including the "mpirun -n 2"as you suggested:
>
> mpirun -n 2 raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAAUTO
> --auto-prot=aicc -n outfile -# 2
>
> I am also obtaining the best tree. Is that ok? the best tree
> reconstruction is using the best model selected?

That should be the case, but I am not 100% sure if this is what the code
does, I will need to check, but it will take some time until I get to this.

Alexis
> <mailto:alexei...@gmail.com
> <mailto:raxml%252Buns...@googlegroups.com
> <mailto:raxml%25252Bun...@googlegroups.com>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>>>>
> <mailto:raxml%252Buns...@googlegroups.com
> <mailto:raxml%25252Bun...@googlegroups.com>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>>>>>.
> <mailto:raxml%252Buns...@googlegroups.com
> <mailto:raxml%25252Bun...@googlegroups.com>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>>>>.
> For more options, visit
> https://groups.google.com/d/optout.
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674
> <tel:56-63-2221674> <tel:56-63-2221674 <tel:56-63-2221674>>
> <tel:56-63-2221674 <tel:56-63-2221674> <tel:56-63-2221674
> <tel:56-63-2221674>>>
> fax: 56-63-2221344 <tel:56-63-2221344>
> <tel:56-63-2221344 <tel:56-63-2221344>> <tel:56-63-2221344
> <tel:56-63-2221344> <tel:56-63-2221344 <tel:56-63-2221344>>>
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate
> <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención Ecología
> y Evolución <http://www.ecolevol.cl>
>
> --
> You received this message because you
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> <mailto:raxml%252Buns...@googlegroups.com>>
> <tel:56-63-2221674 <tel:56-63-2221674>> <tel:56-63-2221674
> <tel:56-63-2221674> <tel:56-63-2221674 <tel:56-63-2221674>>>
> fax: 56-63-2221344 <tel:56-63-2221344>
> <tel:56-63-2221344 <tel:56-63-2221344>> <tel:56-63-2221344
> <tel:56-63-2221344> <tel:56-63-2221344 <tel:56-63-2221344>>>
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate
> <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención Ecología y
> Evolución <http://www.ecolevol.cl>
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674 <tel:56-63-2221674>
> You received this message because you are subscribed to
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>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674 <tel:56-63-2221674>

Juan C. Opazo

unread,
Dec 9, 2015, 6:51:26 AM12/9/15
to raxml
you are right, the names are similar, but anyways, thank you to both of you for helping me with my questions.

best

juan

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--
Juan C. Opazo
Instituto de Cs. Ambientales y Evolutivas
Facultad de Ciencias
Universidad Austral de Chile
Casilla 567, Valdivia
Chile
fono: 56-63-2221674
fax: 56-63-2221344
jopazo (at) gmail (dot) com

OpazoLab

Juan C. Opazo

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Dec 26, 2015, 3:21:58 PM12/26/15
to raxml
Dear Alex, I have the following question, I ran the bootstrap analysis and when I am tried to get the consensus tree using the this command:

 mpirun -n 2 raxmlHPC-MPI -m PROTGAMMAJTT -J MR -z RAxML_bootstrap.outfile -n con

I got this message:

This is RAxML MPI Process Number: 0
Error: you are running the parallel MPI program but only want to compute one tree
For the MPI version you must specify a number of trees greater than 1 with the -# or -N option


This is RAxML MPI Process Number: 1
--------------------------------------------------------------------------
mpirun noticed that the job aborted, but has no info as to the process
that caused that situation.
--------------------------------------------------------------------------


I tried changing the -n to 1, but I got the same message, also if I remove the first portion of the command ( mpirun -n 2).

Do you have any idea of what is going on?

thank you in advance

Juan

Alexandros Stamatakis

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Dec 27, 2015, 1:20:04 AM12/27/15
to ra...@googlegroups.com
you just need to run the consensus option with the sequential or
pthreads version of raxml, the MPI version does not support this,

alexis

On 26.12.2015 21:21, Juan C. Opazo wrote:
> Dear Alex, I have the following question, I ran the bootstrap analysis
> and when I am tried to get the consensus tree using the this command:
>
> mpirun -n 2 raxmlHPC-MPI -m PROTGAMMAJTT -J MR -z
> RAxML_bootstrap.outfile -n con
>
> I got this message:
>
> *This is RAxML MPI Process Number: 0
> Error: you are running the parallel MPI program but only want to compute
> one tree
> For the MPI version you must specify a number of trees greater than 1
> with the -# or -N option
>
> This is RAxML MPI Process Number: 1
> --------------------------------------------------------------------------
> mpirun noticed that the job aborted, but has no info as to the process
> that caused that situation.
> --------------------------------------------------------------------------*
>
> I tried changing the -n to 1, but I got the same message, also if I
> remove the first portion of the command ( mpirun -n 2).
>
> Do you have any idea of what is going on?
>
> thank you in advance
>
> Juan
>
> On Wed, Dec 9, 2015 at 3:51 AM, Juan C. Opazo <jop...@gmail.com
> <mailto:jop...@gmail.com>> wrote:
>
> you are right, the names are similar, but anyways, thank you to both
> of you for helping me with my questions.
>
> best
>
> juan
>
> On Wed, Dec 9, 2015 at 6:56 AM, Alexandros Stamatakis
> <alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>> wrote:
>
> Hi Juan,
>
> Note that Alexey also answered on here (he is my PhD student) so
> it was not only me who helped, I admit the names are pretty similar.
>
> Alexey, I've noted that I need to include a check for #processes < 2
>
> raxmlHPC-MPI -s Alignment.fasta -p 12345 -m PROTGAMMAAUTO
> --auto-prot=aicc -n outfile -# 2
>
> and I got the model, nothing else.
>
> After including the "mpirun -n 2"as you suggested:
>
> mpirun -n 2 raxmlHPC-MPI -s Alignment.fasta -p 12345 -m
> PROTGAMMAAUTO
> --auto-prot=aicc -n outfile -# 2
>
> I am also obtaining the best tree. Is that ok? the best tree
> reconstruction is using the best model selected?
>
>
> That should be the case, but I am not 100% sure if this is what
> the code does, I will need to check, but it will take some time
> until I get to this.
>
> Alexis
>
>
> best
>
> juan
>
>
> On Tue, Dec 8, 2015 at 9:45 PM, Juan C. Opazo
> <jop...@gmail.com <mailto:jop...@gmail.com>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>
>
> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>>>>>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>
>
> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>>>>>>.
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>
>
> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>>>>>.
> For more options, visit
> https://groups.google.com/d/optout.
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs.
> Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674
> <tel:56-63-2221674>
> <tel:56-63-2221674 <tel:56-63-2221674>>
> <tel:56-63-2221674 <tel:56-63-2221674> <tel:56-63-2221674
> <tel:56-63-2221674>>>
> <tel:56-63-2221674 <tel:56-63-2221674>
> <tel:56-63-2221674 <tel:56-63-2221674>> <tel:56-63-2221674
> <tel:56-63-2221674>
> <tel:56-63-2221674 <tel:56-63-2221674>>>>
> fax: 56-63-2221344
> <tel:56-63-2221344> <tel:56-63-2221344 <tel:56-63-2221344>>
> <tel:56-63-2221344 <tel:56-63-2221344>
> <tel:56-63-2221344 <tel:56-63-2221344>>> <tel:56-63-2221344
> <tel:56-63-2221674 <tel:56-63-2221674>>> <tel:56-63-2221674
> <tel:56-63-2221674>
> <tel:56-63-2221674 <tel:56-63-2221674>>
> <tel:56-63-2221674 <tel:56-63-2221674> <tel:56-63-2221674
> <tel:56-63-2221674>>>>
> fax: 56-63-2221344
> <tel:56-63-2221344> <tel:56-63-2221344 <tel:56-63-2221344>>
> <tel:56-63-2221344 <tel:56-63-2221344>
> <tel:56-63-2221344 <tel:56-63-2221344>>> <tel:56-63-2221344
> <tel:56-63-2221344>
> <tel:56-63-2221344 <tel:56-63-2221344>>
> <tel:56-63-2221344 <tel:56-63-2221344> <tel:56-63-2221344
> <tel:56-63-2221344>>>>
> jopazo (at) gmail (dot) com
>
> OpazoLab <http://www.opazolab.cl/>
> ResearchGate
> <https://www.researchgate.net/profile/Juan_Opazo>
> Doctorado en Ciencias mención
> Ecología y
> Evolución <http://www.ecolevol.cl>
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674
> <tel:56-63-2221674> <tel:56-63-2221674 <tel:56-63-2221674>>
> Alexandros (Alexis) Stamatakis
>
> Research Group Leader, Heidelberg Institute for Theoretical Studies
> Full Professor, Dept. of Informatics, Karlsruhe Institute of
> Technology
> Adjunct Professor, Dept. of Ecology and Evolutionary Biology,
> University
> of Arizona at Tucson
>
> www.exelixis-lab.org <http://www.exelixis-lab.org>
>

Juan C. Opazo

unread,
Dec 27, 2015, 4:20:26 AM12/27/15
to ra...@googlegroups.com
Ok, thank you for your answer.

Best

Juan

Sent from my interior
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Juan C. Opazo

unread,
Dec 28, 2015, 7:00:56 AM12/28/15
to raxml
Dear Alex, its me again, I installed RAxML from the repository and I made the consensus tree using the following instructions:

raxmlHPC -m PROTGAMMAJTT -J MR -z RAxML_bootstrap.outfile -n con

I got the following output:
_________________________________________________________________
Found a total of 118 taxa in first tree of tree collection RAxML_bootstrap.outfile
Expecting all remaining trees in collection to have the same taxon set


This is RAxML version 7.2.8 released by Alexandros Stamatakis in October 2010.


With greatly appreciated code contributions by:
Andre Aberer (TUM)
Simon Berger (TUM)
John Cazes (TACC)
Michael Ott (TUM)
Nick Pattengale (UNM)
Wayne Pfeiffer (SDSC)
Akifumi S. Tanabe (Univ. Tsukuba)


RAxML consensus tree computation



RAxML was called as follows:

raxmlHPC -m PROTGAMMAJTT -J MR -z RAxML_bootstrap.outfile -n con


Found 1008 trees in File RAxML_bootstrap.outfile

RAxML Majority Rule consensus tree written to file: XXXXXXXXXX/RAxML_MajorityRuleConsensusTree.con
_________________________________________________________________

Finally I tried to open the tree in FigTree but I got this error message:

Error reading the tree file
Number Format Error: For input string : "1.0[97]"

do you have an idea of what is going on?


thank you in advance


juan

Alexandros Stamatakis

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Dec 28, 2015, 9:23:00 AM12/28/15
to ra...@googlegroups.com
hi juan,

1. you should update to raxml version 8.2.4 available at:
https://github.com/stamatak/standard-RAxML

2. the dendroscope viewer can correctly read this output format

alexis
> >> <mailto:jop...@gmail.com <mailto:jop...@gmail.com>>> wrote:
> >>
> >> you are right, the names are similar, but anyways, thank you
> to both
> >> of you for helping me with my questions.
> >>
> >> best
> >>
> >> juan
> >>
> >> On Wed, Dec 9, 2015 at 6:56 AM, Alexandros Stamatakis
> >> <alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>
> >> <mailto:alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>>> wrote:
> >>
> >> Hi Juan,
> >>
> >> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>
> >> <mailto:raxml%252525252B...@googlegroups.com
> <mailto:raxml%25252525252...@googlegroups.com>>>>>>
> >> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>
> >> <mailto:raxml%252525252B...@googlegroups.com
> <mailto:raxml%25252525252...@googlegroups.com>>>>>>>.
> >> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>
> >> <mailto:raxml%252525252B...@googlegroups.com
> <mailto:raxml%25252525252...@googlegroups.com>>>>>>.
> >> You received this message because you are
> >> subscribed to
> >> a topic in the Google Groups "raxml" group.
> >> To unsubscribe from this topic, visit
> >>https://groups.google.com/d/topic/raxml/xh8DcOMULHw/unsubscribe.
> >> To unsubscribe from this group and all its
> >> topics, send
> >> an email toraxml+u...@googlegroups.com
> >> fax: 56-63-2221344 <tel:56-63-2221344>
> >> <tel:56-63-2221344 <tel:56-63-2221344>>
> >> jopazo (at) gmail (dot) com
> >>
> >> OpazoLab <http://www.opazolab.cl/>
> >> ResearchGate
> >> <https://www.researchgate.net/profile/Juan_Opazo>
> >> Doctorado en Ciencias mención Ecología y Evolución
> >> <http://www.ecolevol.cl>
> >>
> >> --
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> <mailto:raxml%252Buns...@googlegroups.com>
> >> <mailto:raxml%252Buns...@googlegroups.com
> <mailto:raxml%25252Bun...@googlegroups.com>>>
> >>
> <mailto:raxml+un...@googlegroups.com
> <mailto:raxml%2Bunsu...@googlegroups.com>
> >> <mailto:raxml%2Bunsu...@googlegroups.com
> <mailto:raxml%252Buns...@googlegroups.com>>
> >>
> <mailto:raxml%2Bunsu...@googlegroups.com
> <mailto:raxml%252Buns...@googlegroups.com>
> >> <mailto:raxml%252Buns...@googlegroups.com
> <mailto:raxml%25252Bun...@googlegroups.com>>>>.
> >> For more options, visit
> >>https://groups.google.com/d/optout.
> >>
> >>
> >> --
> >> You received this message because you are
> >> subscribed to a topic
> >> in the Google Groups "raxml" group.
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> >> To unsubscribe from this group and all its topics,
> >> send an email
> >> toraxml+u...@googlegroups.com
> >> Juan C. Opazo
> >> Instituto de Cs. Ambientales y Evolutivas
> >> Facultad de Ciencias
> >> Universidad Austral de Chile
> >> Casilla 567, Valdivia
> >> Chile
> >> fono: 56-63-2221674 <tel:56-63-2221674>
> >> fax: 56-63-2221344 <tel:56-63-2221344>
> >> jopazo (at) gmail (dot) com
> >>
> >> OpazoLab <http://www.opazolab.cl/>
> >> ResearchGate <https://www.researchgate.net/profile/Juan_Opazo>
> >> Doctorado en Ciencias mención Ecología y Evolución
> >> <http://www.ecolevol.cl>
> >>
> >> --
> >> You received this message because you are subscribed to the
> >> Google
> >> Groups "raxml" group.
> >> To unsubscribe from this group and stop receiving emails
> >> from it, send
> >> an email toraxml+u...@googlegroups.com
> >> For more options, visithttps://groups.google.com/d/optout.
> >>
> >>
> >> --
> >> Alexandros (Alexis) Stamatakis
> >>
> >> Research Group Leader, Heidelberg Institute for Theoretical Studies
> >> Full Professor, Dept. of Informatics, Karlsruhe Institute of
> >> Technology
> >> Adjunct Professor, Dept. of Ecology and Evolutionary Biology,
> >> University
> >> of Arizona at Tucson
> >>
> >>www.exelixis-lab.org <http://www.exelixis-lab.org>
> <http://www.exelixis-lab.org>
> >>
> >> --
> >> You received this message because you are subscribed to a topic
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> >> For more options, visithttps://groups.google.com/d/optout.
> >> an email toraxml+u...@googlegroups.com
> >> For more options, visithttps://groups.google.com/d/optout.
> >
> > --
> > Alexandros (Alexis) Stamatakis
> >
> > Research Group Leader, Heidelberg Institute for Theoretical Studies
> > Full Professor, Dept. of Informatics, Karlsruhe Institute of Technology
> > Adjunct Professor, Dept. of Ecology and Evolutionary Biology, University
> > of Arizona at Tucson
> >
> >www.exelixis-lab.org <http://www.exelixis-lab.org>
> >
> > --
> > You received this message because you are subscribed to a topic in the Google Groups "raxml" group.
> > To unsubscribe from this topic, visithttps://groups.google.com/d/topic/raxml/xh8DcOMULHw/unsubscribe.
> > To unsubscribe from this group and all its topics, send an email
> to raxml+un...@googlegroups.com
> <mailto:raxml%2Bunsu...@googlegroups.com>.
> > For more options, visit https://groups.google.com/d/optout.
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674

Juan C. Opazo

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Dec 28, 2015, 4:49:15 PM12/28/15
to raxml
It works! thank you very much!
--
Juan C. Opazo
Instituto de Cs. Ambientales y Evolutivas
Facultad de Ciencias
Universidad Austral de Chile
Casilla 567, Valdivia
Chile
fono: 56-63-2221674
fax: 56-63-2221344
jopazo (at) gmail (dot) com

OpazoLab

Juan C. Opazo

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Jan 20, 2016, 7:39:48 AM1/20/16
to raxml
Hi Alex, I have a question for you regarding the-i parameter. You mention that the value of this parameter is determine automatically by raxml, but it is possible to know the value assigned? Can you guide me regarding the values should I use? I guess a higher number means that the exploration of the tree space is better? Am I right?

thank you in advance

juan 

Alexandros Stamatakis

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Jan 21, 2016, 4:09:39 AM1/21/16
to ra...@googlegroups.com


On 20.01.2016 13:39, Juan C. Opazo wrote:
> Hi Alex, I have a question for you regarding the-i parameter. You
> mention that the value of this parameter is determine automatically by
> raxml, but it is possible to know the value assigned?

it should be printed out in the RAxML_info file as "rearrangement
radius" or something similar ...

> Can you guide me
> regarding the values should I use?

How to set up these values by hand is outlined in the RAxML manual ..

> I guess a higher number means that
> the exploration of the tree space is better? Am I right?

yes, that's correct,

alexis

>
> thank you in advance
>
> juan
>
> On Mon, Dec 28, 2015 at 6:48 PM, Juan C. Opazo <jop...@gmail.com
> <mailto:jop...@gmail.com>> wrote:
>
> It works! thank you very much!
>
> On Mon, Dec 28, 2015 at 6:22 AM, Alexandros Stamatakis
> <alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>> wrote:
>
> hi juan,
>
> <mailto:jop...@gmail.com <mailto:jop...@gmail.com>>> wrote:
>
> Ok, thank you for your answer.
>
> Best
>
> Juan
>
> Sent from my interior
>
> > On Dec 27, 2015, at 3:19 AM, Alexandros Stamatakis
> <alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>
> >> <mailto:jop...@gmail.com <mailto:jop...@gmail.com>
> <mailto:jop...@gmail.com <mailto:jop...@gmail.com>>>> wrote:
> >>
> >> you are right, the names are similar, but
> anyways, thank you
> to both
> >> of you for helping me with my questions.
> >>
> >> best
> >>
> >> juan
> >>
> >> On Wed, Dec 9, 2015 at 6:56 AM, Alexandros
> Stamatakis
> >> <alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>
> <mailto:alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>>
> >> <mailto:alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>
> <mailto:alexandros...@gmail.com
> <mailto:alexandros...@gmail.com>>>> wrote:
> >>
> >> Hi Juan,
> >>
> <mailto:raxml%2525252Bu...@googlegroups.com>>
> >>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>
> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>>>>>
> <mailto:raxml%2525252Bu...@googlegroups.com>>
> >>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>
> <mailto:raxml%2525252Bu...@googlegroups.com
> <mailto:raxml%252525252B...@googlegroups.com>>>>
> <mailto:raxml%25252Bun...@googlegroups.com
> <mailto:raxml%2525252Bu...@googlegroups.com>
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674 <tel:56-63-2221674>
> fax: 56-63-2221344 <tel:56-63-2221344>
> jopazo (at) gmail (dot) com
>
> Doctorado en Ciencias mención Ecología y Evolución
> <http://www.ecolevol.cl>
>
>
>
>
> --
> Juan C. Opazo
> Instituto de Cs. Ambientales y Evolutivas
> Facultad de Ciencias
> Universidad Austral de Chile
> Casilla 567, Valdivia
> Chile
> fono: 56-63-2221674
> fax: 56-63-2221344
> jopazo (at) gmail (dot) com
>
> Doctorado en Ciencias mención Ecología y Evolución <http://www.ecolevol.cl>
>
> --
> You received this message because you are subscribed to the Google
> Groups "raxml" group.
> To unsubscribe from this group and stop receiving emails from it, send
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