warnings.warn("Mean of empty slice.", RuntimeWarning)

[Mori Giorgia]

Hi,

I am new to qiime and trying to demultiplex a set of reads from 9 different samples.
If I use

split_libraries_fastq.py -i Sam1-9_S10_L002_R1_001.fastq -o demult -m 022717MRP515F-mapping.txt -b barcodes.fastq  --barcode_type 8

this is what it comes

/home/mycolab4/anaconda3/envs/qiime1/lib/python2.7/site-packages/numpy/core/_methods.py:59: RuntimeWarning: Mean of empty slice.
  warnings.warn("Mean of empty slice.", RuntimeWarning)


I cannot see where I am doing wrong,

can anybody help me please?

Thank you,

Giorgia

[Jenya Kopylov]

Hi Giorgia,

Have you looked through the output logs for errors? Based on other QIIME posts with this error, the issue could be with your barcodes, see:

Post 1: split_libraries_fastq.py error: warnings.warn("Mean of empty slice.", RuntimeWarning)

Post 2: Split libraries: "Mean of empty slice.", RuntimeWarning

Post 3: split_libraries_fastq.py RuntimeWarning: Mean of empty slice

Jenya