Interpreting Score for Neuron against GAl4 Lines.

[George Yerou]

Dear Dr Jefferis/ NBLAST community.

I have found several morphologically similar FlyCricuit neurons, all of which score relatively highly for a GAL4 line on NBLAST  (between 0.37-0.47 depending on the Neuron). Does this indicate that all the neurons are driven by that line or does it means only one of them is driven by that line and the others score as product of them having similar morphology to it?

Help is greatly appreciated!

Best Wishes,

George.

[Greg Jefferis]

Dear George,

On 11 Oct 2020, at 18:02, George Yerou <gj.y...@gmail.com> wrote:

I have found several morphologically similar FlyCricuit neurons, all of which score relatively highly for a GAL4 line on NBLAST  (between 0.37-0.47 depending on the Neuron). Does this indicate that all the neurons are driven by that line or does it means only one of them is driven by that line and the others score as product of them having similar morphology to it?

Help is greatly appreciated!

I am afraid there cannot be an absolute rule here. Your first question might be: could the FlyCircuit neurons all be examples of the same cell type? Your second might be whether the FC neurons have any anatomical features incompatible with the GAL4 line. For GMR GAL4 lines, you may find the new multicolour fly out (MCFO) data useful. 

https://gen1mcfo.janelia.org/

In evaluating the constituent neurons of GAL4 lines. Note also that the neuronbridge website provides a very effective way to do certain searches including hemibrain neurons vs GMR Gen 1 MCFO data.

All the best,

Greg.

[gj.y...@gmail.com]

Dear Dr Jefferis,

 

Thank you for your help! I would further like to clarify why some of the hits returned using neuron bridge seem to bear little resemblance to the Query. I uploaded the following image as a query:

Bellow are images including the top LM and EM matches for the image:

On the Right hand side in both images, a descending neuron akin to the one in the query is prominent and it is clear to see how the expression patterns are related. On the left hand side, the expression patterns and neuron look unrecognisable from the query.

Thank you for your time.

 

Best wishes,

George.

[Greg Jefferis]

Dear George,

I'm not sure that I can offer any real insight, certainly without access to your upload. I think you would really need to contact the neuronbridge developers. However I would be very careful to check that your uploaded image matches the required dimensions etc. The scores you are getting strike me as on the low side even if the DNp27 hits are real.

With best wishes,

Greg.