Hi Ahmed,
We're happy to help with specific questions about using the MEME Suite, but this question is so general that it's not answerable in a few paragraphs. I'd direct you to the
documentation for the MEME Suite command line tools. You'll also find some brief summaries here on the MEME Suite Q&A site:
For more detailed information, you may also want to review some of the papers published for these tools. You can find a list of the relevant papers
here.
If you aren't familiar with using the UNIX/Linux command line you'll need to find a local mentor, or review one of the many online introductions. Assistance with basic command line usage is beyond the scope of help we can offer.
Finally, I would offer that MAST is not a tool for genome scale analysis. This question on the MEME Suite Q&A site may be of help:
If you have specific questions after reviewing these materials, we'll be happy to try and help.