.gb annotations all showing incorrect start positions at coordinate 1

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kevi...@stanford.edu

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Oct 1, 2017, 5:43:07 AM10/1/17

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Dear IGV team-

version = IGV 2.4.1

OS = macOS Sierra 10.12.6

I loaded a genbank file (pKR291.gb) into IGV (using Genomes -> Load Genome from File...). The coordinates for the all of the annotations start at position 1 when they should start at various coordinates throughout the plasmid. Each annotation shows the correct stop coordinate; only the start positions are affected. The .gb file was generated by Benchling (attached) and when re-loaded into Benchling appears normal. This occurs for other .gb files as well (pKR326.gb for example), while other .gb files load fine (such as pKR194.gb). I am attaching all of these .gb files to see if you can replicate this issue with my files. The screenshots for pKR291 and pKR194 plasmid annotations in both IGV and Benchling are also attached.

Thanks in advance for your help!

Kevin

pKR291.gb

pKR326.gb

pKR291_Benchling_snapshot.png

pKR194_Benchling_snapshot.png

pKR194_igv_snapshot.png

pKR291_igv_snapshot.png

pKR194.gb

igv.log

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