Equal signs on reads

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fvigoder

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Mar 26, 2018, 3:27:22 PM3/26/18
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Hi,
I`m having an issue visualizing reads sequences in IGV.

I`m trying to see the sequence of reads but many are displaying = instead of the bases. 
What does the = means?
Is it an error from the alignment?

James Robinson

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Mar 26, 2018, 10:03:06 PM3/26/18
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Its a convention allowed in the SAM/BAM specification,  I don't often see it used.   Here's what the spec document says

An `=' denotes the base is identical to the reference base.

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