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More specifically here: https://downloads.yeastgenome.org/sequence/strains/SK1/SK1_SGD_2015_JRIH00000000/Genome (fasta)Annotations:You will need to gunzip the fasta before loading into IGV. Then use "Genomes > Load genome from file". After its loaded you can load the annotations directly from "File > Load from file"
On Fri, Sep 1, 2017 at 3:57 PM, James Robinson <jrob...@broadinstitute.org> wrote:
Hi,Unfortunately I don't have an sk1 fasta file, this genome is an early addition to IGV and at the time we used a non-standard format. You should be able to download the sequence as a fasta from here: https://www.yeastgenome.org/strain/SK1
On Fri, Sep 1, 2017 at 1:36 PM, Berchowitz Lab <bercho...@gmail.com> wrote:
Hello-My name is Luke Berchowitz and I am an assistant professor at Columbia University Medical Center.We recently did an experiment in which we purified an RNA-binding protein from S. cerevisiae (SK1 strain) and sequenced the associated RNA. I need to map the reads and the reference genome available on yeastgenome.org is faulty. I have noticed that IGV has a fully annotated SK1 sequence option.Would it be possible for you to share with me the SK1 reference files so that I can map our reads and then view them on IGV? We would greatly appreciate it.Thanks and best regards,Luke
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Try this
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