SYBRGreen Datasheet

[Gabriele Borelli]

Hello,

I am studying Electronic Bioengineering at University of Bologna. I am beginning to study the working principle of qPCR, more specific I am now focusing on SYBRGreen. I would like to do some optical tests on that chemical compound. My biological knowledges are not excellent, so I thought that my first step could be finding a datasheet of this subastance. I did several researches on google, but I can't find any documents where the behavior of the SYBRGreen is completely described. I would like to know:

- emitted light / incident light

- wavelenght of the emitted light

- sensibility / wavelength of the incident light

- changes with temperature (?)

So I would like to have a datasheet, something that describes SYBRGreen entirely.

Where can I find this document?

Thanks and regards,

Gabriele

[Nathan McCorkle]

This should be helpful:
http://www.theinstituteoffluorescence.com/Publication%202/JoFL%20paper.pdf

I searched google scholar with these keywords:
'sybr chemistry fluorophore chromophore'

That link was the 4th result.

> --
> -- You received this message because you are subscribed to the Google Groups
> DIYbio group. To post to this group, send email to diy...@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+un...@googlegroups.com. For more options, visit this group at
> https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to diybio+un...@googlegroups.com.
> To post to this group, send email to diy...@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio.
> To view this discussion on the web visit
> https://groups.google.com/d/msgid/diybio/9f609bc3-7eb0-4d02-96ac-7cc34a31762d%40googlegroups.com.
> For more options, visit https://groups.google.com/d/optout.



--
-Nathan

[Gabriele Borelli]

Hello,

thank you for your help. I read the documents, but, as on every document I can find on the web, there are only normalized values of intensity. 

I know that if I put together SYBR Green and DNA the fluorescence increase by 1000 times, but how much light does it emits respectively to the one that hit the molecole?

I mean that I want to understand if I can measure that fluorescence with a sensor that for example has this curve of sensitivity:

http://s24.postimg.org/bjc1ifhyd/Capture.png

So I would to know the order of magnitude of this fluorescence (that obviously is related to the intensity of light with which I hit the SYBR Green).

Thanks and regards,

Gabriele

[Nathan McCorkle]

On Thu, Mar 5, 2015 at 10:53 AM, Gabriele Borelli <borel...@gmail.com> wrote:
> Hello,

> thank you for your help. I read the documents, but, as on every document I
> can find on the web, there are only normalized values of intensity.
> I know that if I put together SYBR Green and DNA the fluorescence increase
> by 1000 times, but how much light does it emits respectively to the one that
> hit the molecole?

You would need to look-up the quantum efficiency I believe.

[Matt Lawes]

You can probably find quantum yield / efficiency data for SYBR Green at molecularprobes.com. It is their dye.

>matt

Sent from my Verizon Wireless 4G LTE DROID

Nathan McCorkle <nmz...@gmail.com> wrote:

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9Jt1T7uVgsSJR0Kfim9cyy5PoauS-8jCzRk5XW-ciaDug%40mail.gmail.com.