DIY stem cell extraction from teeth's pulp

[Mega [Andreas Stuermer]]

Hi everyone,

I was wondering whether anyone knows a good method how to get CD105+ stem cells from wisdom teeth pulp in a diy setting... Like described in here   "Complete Pulp Regeneration After Pulpectomy by Transplantation of CD105+  Stem Cells with Stromal Cell-Derived Factor-1" (I can send the paper to anyone interested).
Short version: IN dental pulp there are stem cells which can be used to re-grow nerves, blood vessels, etc.


I know, concerns have been told about breeding viruses in a diy setting. But after like 10 divisions, how many viruses can be bred?  Btw, (off-topic) noone ever had the idea to transfect mammalian cells with crispr which kills all the viruses?
Note my disclaimer, I don't want to engineer them, just purify them and have them grow for a few cell divisions.

[Nathan McCorkle]

I think you'll at least need some serum, or look into serum-free media. I thought about trying a diy cell culture of pig heart when my farmer next sells a pig for meat. That way I could centrifuge some blood for serum, then filter it, and try to come up with suitable drug-store replacement ingredients for the salts/ions/vitamins/carbs etc.

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[Nathan McCorkle]

Viruses basically fill up the internals of a cell so much that the cell bursts (if the virus isn't dormant) so there will be a ton of virus particles.

Best to try doing any of that work in a laminar flow hood, but if not, make sure you don't prick yourself during work, always wash your hands and arms after work, don't atomize or spill culture, maybe even wear a face mask that deals completely around your face (this will help keep you and the culture clean).

On Jul 11, 2014 5:59 AM, "Mega [Andreas Stuermer]" <masters...@gmail.com> wrote:

[Andreas Stuermer]

Btw, by adding zinc to the medium some viruses are severely inhibited or cannot attach to the cells surface.

Is there a way to get CD105+ cells without an expensive FACS?

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[Andreas Stuermer]

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC86580/

http://www.ncbi.nlm.nih.gov/pubmed/2177090/

On Fri, Jul 11, 2014 at 9:27 PM, Nathan McCorkle <nmz...@gmail.com> wrote:

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