Auxotroph yeast strain

Andreas "Mega" Stuermer

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Dec 18, 2018, 6:15:41 PM12/18/18

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After finding a Yeast artificial chromosome that will work and is not bound by copyrights, I realized I will need a yeast strain auxotrophic for Leucin biosynthesis :D
By the way, if anyone needs it, will share.

Anyone with access to a yeast lab strain?

Seth Donnelley

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Dec 19, 2018, 8:18:38 PM12/19/18

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No, but you can try using CRISPR to knock out leucine aminotransferase.

Seth

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Andreas "Mega" Stuermer

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Dec 22, 2018, 2:53:55 PM12/22/18

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Great idea, but then you might have to delete some other recombinase protein too to ensure stability?

Seth Donnelley

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Dec 22, 2018, 5:23:21 PM12/22/18

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Yes, I suppose so. Hmm...

On Sat, Dec 22, 2018 at 2:54 PM Andreas "Mega" Stuermer <andreas.t...@gmail.com> wrote:

Great idea, but then you might have to delete some other recombinase protein too to ensure stability?

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