My DIY Gene Gun worked!!

[Sebastian Cocioba]

Here is a picture of a plant leaf expressing gfp shot using tungsten nanoparticles using my home made gene gun. Ill write a decent post on my blog about it but I was too excited to wait. This was 48hrs post shot. Tobacco leaf, 35s-gfp-Tnos cassette. Left leaf is experiment, right leaf is control. Not the glow in the midrib. Leaf illuminated with party light black light.

Cost of gene gun was $30-50 depending on shipping (plumbing parts and bike pump), tungsten is $38/5g from US Nano, not counting the calcium or spermidine on looking at $0.01/shot (0.5mg) and all done at room temp!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

[Cathal Garvey (Phone)]

WHOA Yes! Congrats Sebastian, can't wait to build one!

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[Alex]

Well done!

Alex

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[Mega [Andreas Stuermer]]

Very awesome! 

U should sell them ;)

[Avery louie]

Congrats!

On Sat, Apr 19, 2014 at 3:08 PM, Mega [Andreas Stuermer] <masters...@gmail.com> wrote:

Very awesome! 

U should sell them ;)

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[Meredith L. Patterson]

Sweet! Can't wait to read the writeup! (And if it's just plumbing parts and a bike pump, shit, I probably have all that lying around...)

Cheers,

--mlp

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[Alexey Zaytsev]

Hey. Super-nice.

Just one thing. Do you have a control? Because it really looks like some fluorescent dust fibers.

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[Sebastian Cocioba]

Control is the right leaf. Dust is a big issue when looking for false positives. That's why I aimed at the midrib. I wiped off any dust but tobacco is sticky. The midrib color is the best signal since I shot into leaf with poor results.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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From: Alexey Zaytsev
Sent: ‎4/‎19/‎2014 3:38 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] My DIY Gene Gun worked!!

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[Simon Quellen Field]

The "dust" looks to me like lint from fabric that has been treated with "whitening agents", which are just dyes that fluoresce blue. They are added to detergents because as cotton ages, it yellows, and yellow plus blue look white.

Adding a low pass filter would block the blue and let the green show through.

Or you could remove them digitally, by erasing blue pixels.

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[Simon Quellen Field]

By the way, fabric whitening agents may soon fail to work indoors:

"http://gizmodo.com/led-lights-are-ruining-laundry-detergents-white-brighte-1565070690"

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[Simon Quellen Field]

I took Sebastian's photo and set the brightness, contrast, and gamma of the red and blue to zero.

In the result, the bright blue lint dust has nearly disappeared.

​To make the difference between the control and the subject pop out a little more, I converted it to grayscale:

​Lastly, I selected only the brightest tail of the distribution, to remove clutter:

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On Sat, Apr 19, 2014 at 6:03 PM, Simon Quellen Field <sfi...@scitoys.com> wrote:

[Sebastian Cocioba]

Wow that looks fantastic! Could I use those images in my write-up and credit you for the image manipulation?

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

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From: Simon Quellen Field
Sent: ‎4/‎20/‎2014 4:29 PM
To: diybio

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[Rikke Rasmussen]

Sebastian, this is amazing! I can't wait for the write-up. Is there any way I can persaude you to do an instruction video on your assembly?

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[Mac Davis]

I'll do an instructional video based on the write-up within the week after it comes out.

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