Extracting substances from a chromotography paper

[Aubrey]

So if I were to extract amino acids by hydrolyzing human hair, and then separated them out with chromotography paper, then identified each one according to reference Rf values, could I then be able to then remove the amino acids from the paper and convert them to their powdered form?

I had an idea that if I cut up the paper (separating each type of amino acids) and then dunk it in pure water the amino acids dissolve into the water and I can then remove the paper and allow the water to evaporate leaving the amino acids behind. 

Thoughts?

[Koeng]

Yes, but you'd likely get trace quantities that aren't all that useful. What is the goal?

[Aubrey]

I hope to be able to use the amino acids in a tissue culture (yes I know a.a. comes in media but I want to add my own concentrations and I know I can buy amino acid supplements but I'm trying to save money)

[Abizar Lakdawalla]

Hi Aubrey, you need 100s mg/L for tissue culture. Hair hydrolysis also requires a lot more effort. Egg white might be a better starting point. TLC wont produce enough materials though, you might have to go to column chromatography but then buying amino acids turns out to be much cheaper. Mammalian tissue culture media is not that expensive, 500 ml costs about $30.

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