Yeast promoter engineering

[Nico B.]

Hey all!

I want to get my hands wet on yeast engineering. Has anyone ever used the TEF1 promoter from Ashbya gossypii? I want to use two or three promoters, but cannot take the same twice because of homologous recombination TEF1 from sachhaomyces and TDH3 are on my list. I need a promoter to be constitutively active, but most importantly active on glucose+ oxygen+

Thanks!!

[Francis Lee]

pTEF is a pretty strong promoter; some other good ones are pCYC1 and pADH1.

Also, check out the attached pub from the Deuber lab from 2013.

-F

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