Question about centrifugation???

[Experiments in prebiotic chemistry]

Hi,

  I have a question about centrifugation.  I was wondering, if you centrifuge living cells, let's say oocytes for example, and you end up with a cell extract, wouldn't that cell extract be considered to be dead?  Doesn't centrifugation kill a living cell?

[Cathal Garvey]

It depends on how hard you centrifuge them. For larger and more complex cells, you centrifuge at a lower speed/force, and they tend to settle faster anyway. But for smaller, hard-walled, anucleic cells like bacteria and archaea, you can often centrifuge at full power and no harm done.

Bluntly, if you centrifuge a wall-less oocyte at 13,000rpm in a microcentrifuge then yes, you'll probably end up with cell extract. :)

On 18 February 2020 00:11:07 GMT+00:00, Experiments in prebiotic chemistry <jspr...@gmail.com> wrote:

Hi,

  I have a question about centrifugation.  I was wondering, if you centrifuge living cells, let's say oocytes for example, and you end up with a cell extract, wouldn't that cell extract be considered to be dead?  Doesn't centrifugation kill a living cell?

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Sent from my Android device with K-9 Mail. Please excuse my brevity.