plasmid extraction with "boiling method" ?

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Walter

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Sep 13, 2016, 12:00:37 PM9/13/16
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Hello,

I had try to extract a plasmid form e.coli with the “boiling method” (STET-Buffer and Lyzozyme). I have used 94 % ethanol to precipitate the DNA, but I could centrifuge with 6000 rpm only.

In a following agarose gel electrophoresis I couldn’t see band.

Can everyone help me ?



- Walter
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