Why is my Gluconobacter Agar not sticking to my petri dish?

[Philipp Kollenz]

Hi there,

My Gluconobacter Agar (http://www.thelabrat.com/protocols/GluconobacterAgar.shtml) won't stick to my petri dish. After drying it gets watery again and shrinks about 10% in diameter. When I try to turn the dish around, it just falls of. Potato dextrose agar just works fine with the same procedure, what am I doing wrong?

[Cathal Garvey]

Hmm, it's possible that the mild settling you expect for CaC03 in the
gluconobacter agar reduces the adherence of the gel to the bottom of the
agar plate, so it dislodges sooner than other agars would.

Given the long culture-times you need to observe Gluconacetobacter
xylinus making cellulose, perhaps drying prior to culture isn't
necessary or even advisable? Unless it's dripping wet, streak straight
onto the place and flip to face-downwards carefully to avoid runny cells
from spreading where they're not wanted.

You don't need to raise G.xylinus on gluconobacter agar except when
isolating it, it'll grow happily on many other simple agars, so don't
feel tied to this basic differentiating agar once you have a pure strain.

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[Angel Regalado]

Weed beer what's wrong with this idea?

---

From: Cathal Garvey
Sent: ‎7/‎7/‎2015 12:47 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] Why is my Gluconobacter Agar not sticking to my petridish?

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