DIY Laminar flow hood

[Matt Brown]

I'm trying to put together the equipment I need to start on a DIYBio project, and I was wondering if anyone had some advice on putting together a laminar flow hood for sterile work?  I have plenty of space to set it up, but I not sure what's the best way to construct it or what kind of filters to use.  Any help would be appreciated.

[John Griessen]

On 07/13/2017 01:34 AM, Matt Brown wrote:
> I was wondering if anyone had some advice on putting together a laminar flow hood for sterile work?

Sebastian has some opinions on this.

https://groups.google.com/forum/?utm_medium=email&utm_source=footer#!searchin/diybio/hepa|sort:relevance/diybio/9-A-EHhJxN4/PYVnNiUrMK8J

There's lots discussed here that you find by searching
https://groups.google.com/forum/?utm_medium=email&utm_source=footer#!searchin/diybio/laminar$20flow%7Csort:relevance

https://groups.google.com/forum/?utm_medium=email&utm_source=footer#!searchin/diybio/hepa%7Csort:relevance

I'll be developing a UPS shippable low cost positive pressure HEPA flow bench to sell in a while -- too busy with the
culture shock electroporator right now though. (blog.kitmatic.com and https://cibolo.us/mailman/listinfo/open_electroporator)

The most important things seem to be:

1. Avoid vortices in air flow by using squirrel cage fans, baffles, perforated flow source walls,
straight path out the front door.

2. Use a front screen that raises and lowers to reduce the air flow needed, and that is clear see through and cleanable.

3. Make a control for air flow that adjusts to a good low flow that won't blow on your subjects,
dry them out, blow them around, etc.

4. Make a perfect seal to your HEPA filter element edges.

5. Have decent light sources built in because you cannot snake them in and have good cleanliness.

6. Add other niceties like indicators, carry handles