Tecan EVO2 dispensing variation

[Paul Twyman]

Hi All, I hope everyone is well.

I have an aged Tecan EVO 2 that is causing concern. Each morning it needs to be flushed several times before a reliable dispensing check can be achieved. We are running DMSO. I have replaced all syringes, 3 way valves and tips. Is there anything else that I should check or replace? Thank you in advance for any advice.

Paul

[John Griessen]

On 10/26/20 8:53 AM, Paul Twyman wrote:
> We are running DMSO. I have replaced all syringes, 3 way valves and tips. Is there anything else that I should check or replace?

Since DMSO is a good solvent for oil based, and makes some materials swell up or dissolve, you may have deterioration of some
plastic fittings and leaking.
Is the rest of the piping path made of HDPE or Teflon or PP? If not, the parts made of anything more reactive could be
cracked/leaking.

Another way to analyze this is -- perhaps the new seals in the syringes are being affected by the DMSO while doing initial tests,
then after swelling they are stable. Try to purchase syringes with seals that are unaffected by DMSO.

--
John Griessen

[Dakota Hamill]

Try it with water for a few runs/hours/day to remove DMSO as the variable.  If it works fine with water, DMSO's solvent properties are the issue as John mentioned.  If with water, you still are getting non-accurate volume dispensing, it's not just the solvent. 

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