Spare pOpen Taq plasmids anyone?

[Open BioLab Graz - Austria]

Hi!

Since the openbiotech guys arent answering to our emails since weeks, we thought we try it here:

Does anyone here have some spare pOpenTaq plasmids and would be so kind to send them to our biohacking space in Austria/Europe? We would really like to test our nice equipment :)

[Josiah Zayner]

I don't have pOpenTaq but I have a plasmid that contains Taq polymerase with a his tag so it can be purified on a nickel column. The expression is inducible with IPTG.

[Marc Dusseiller]

hei graz,

we have just recently ordered and received it to be used during "Hack-a-Taw" hacksprint 2 weeks ago in lausanne.

see some info here (also has some nice tests our our self-made PCR thermocyclers...)

http://hackteria.org/?p=2141

we still have some of the plasmids and reagents. kris from BioTehna lab in Ljubljana also took some down. maybe you should come for a visit to LJ anyway, just 1.5 h drive.

best,

m

[Koeng]

I would, but I haven't gotten around to cloning a bunch of it :) If no one else can get it to you, I could probably send some in a week or two

[Open BioLab Graz - Austria]

Thank you for your kind offer Josiah! We have luckily found someone nearby with pOpenTaq.

[Open BioLab Graz - Austria]

Hallo,

wow nice hacksprint. Is there a detailed description of the wild openpcr somewhere? We are also building our own pcr machine right now. 

Yeah we should definitly visit the people in ljubljana, currently we are quite self focused to get our stuff working...

I contacted sachiko, i think he is in your team :)

[Open BioLab Graz - Austria]

Thank you too Koeng! Although we found already a source, dont let this stop you from cloning them ;)

[Ivan Ivanov]

Hi there, I have also tried to buy some of the pOpenTaq plasmid but no answer from the guys from Openbiotech. If someone can send some in Bulgaria/Europe that would be great ! Thanks

[Chengcang Wu]

On Thursday, April 18, 2013 at 10:12:27 AM UTC-5, Josiah Zayner wrote:

I don't have pOpenTaq but I have a plasmid that contains Taq polymerase with a his tag so it can be purified on a nickel column. The expression is inducible with IPTG.

 Hi Josiah,

Could you please share a little of your Tag polymerase plasmid with us?

Thanks,

Chengcang Wu

1100 Corporate Square Dr Suite 257

St Louis, MO 63132

[Josiah Zayner]

Hey Chengcang,

     I have it in a strain but understand it has not been used or tested in years so have small amounts of caution. Iw ill send out the strain in the next few days.

Josiah

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[Chengcang Wu]

Hi Josiah,

We are waiting to receive and test your Taq polymerase. 

Thanks

Chengcang

[Josiah Zayner]

Hey, sorry, totally forgot about this. Will ship it out by tomorrow.

[Андрей Роман]

Hello

Does anyone here have some spare pOpenTaq plasmids and would be so kind to send them to Marseille?

[Seeda]

Hello,

Could someone provide us with pOpenTaq plasmid?

[jarlemag]

Hi,

Gene and Cell Technologies sells pOpentaq for $99: http://www.geneandcell.com/collections/open-source/products/taq-polymerase-plasmid

Best regards,

JP