need to find a gel box capable of 96 samples

[Jeswin]

It's really frustrating trying to find a gel casting tray and box that
can (a) run at least 96 samples, basically a whole 96-well plate, (b)
is useable with multi-channel pipette, and (c) doesn't cost $600+. The
wells don't have to be 8x12. I'm not running a ladder, rather a single
standard band to estimate concentration, so resolution (run length) is
not very important.

I'm trying ebay but its tough because most don't have combs (and not
sure if new ones are available) or fit the above criteria. Some help
please? Thanks

--
In necessariis unitas, in dubiis libertas, in omnibus caritas.
-Marco Antonio Dominis

[scoc...@gmail.com]

Access to a 3D printer to make a quick and dirty custom one? Just make a boat to hold multiple 8 well combs and distribute them across to gel length? I've not seen a commercial 96-well before but certain it exists.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D

---

From: Jeswin
Sent: ‎2/‎24/‎2015 4:10 PM
To: diy...@googlegroups.com
Subject: [DIYbio] need to find a gel box capable of 96 samples

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[Jeswin]

There is one 96 well one, but it's about $500. Like I said, I can go
with 4x25, 8x25, etc. as long as I can use a multipipette. Not having
much luck on ebay, labx, go-dove either. I don't have access to a 3D
printer and I don't know how to use one.

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[John Griessen]

On 02/24/2015 03:10 PM, Jeswin wrote:
> I'm trying ebay but its tough because most don't have combs (and not
> sure if new ones are available) or fit the above criteria. Some help
> please?

Buy a tray, then "make" a comb at a tech shop with a laser cutter...

But then it still needs an electrode...are you OK with stainless steel?

But, you're really asking for a lower price for the one from years ago:

http://blog.ginkgobioworks.com/2009/09/01/pearl-biotech-open-gel-unboxing/

and is it even for sale?

This is the only product now available from pearl:

http://pearlbiotech.com/pages/blue-transilluminator-factsheet

no mention of the old gel box and no "open source" files either... hmmm..

These are now it seems:
http://www.iorodeo.com/content/mini-gel-electrophoresis-system-0

but mini.

[John Griessen]

On 02/24/2015 03:28 PM, Jeswin wrote:
> I don't have access to a 3D
> printer and I don't know how to use one.

I've got one to use at a shared shop I belong to.
What is your budget and how soon is your need, and specs?
Should it be 8 lanes wide or 12? How deep a reservoir on each end -- I think the big 8 - 10 cm depth
of the designs we have seen makes them extra bulky and for what?
Do you need super long run times and a big reservoir?

John

[Jojobead]

That's what I was thinking... building one:  http://teach.genetics.utah.edu/content/build_gel_box.pdf

[John Griessen]

On 02/24/2015 03:43 PM, John Griessen wrote:
> On 02/24/2015 03:28 PM, Jeswin wrote:
>> I don't have access to a 3D

I meant to say laser cutter. 3DP i soften porous and leaky, so not gel box material....yet.

[Nathan McCorkle]

sounds like a robot liquid handler and capillary electrophoresis would
work well.

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--

-Nathan

[Tom Eberhard]

I'd be interested in manufacturing this, can you provide some kind of first pass at a design design?
I have access to laser cutters, milling machines, 3D printers, etc.
Tom.
Alva Bio.