Dear plant DIYers,
I am gearing up towards a plant DIY operation, and I was hoping you could answer a couple of questions that I am having a hard time finding on the internet.
1. Do calluses need light to grow? Some protocols grow them in the dark, so I am confused. If so, what lights/cycle do you recommend? My purpose for for growing calluses (calli?) would be two-fold: somatic embryogenesis, and transformation with Agrobacter in Crassula.
2. Can I use parafilm to seal the callus-growing petri dishes? What about gas exchange?
3. Would the PPM in the callus media kill Agrobacter?
many thanks in advance!
Patricia