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https://www.wyzerbio.com/wiser/#aboutUs has been extraordinarily kind to my friend and I.
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On Jan 9, 2015 10:28 PM, "Patrik D'haeseleer" <pat...@gmail.com> wrote:
> It'd be great if there was some service where you can order just a single bacterial genome sequenced, library generation and all. Most NGS sequencing facilities aren't going to want to put up with the overhead of selling 1/96th of a lane though...
It wouldn't be a bad thing to get 96X though, or at least get that price to do order pooling. Ideally the pooled order would still get library generation treated on their end too. But the right person could pick that up as part of the pooling and as source of income if they could do the prep reliably.
Can you explain what you mean by a single MiSeq lane? Our lab has Life
Tech Ion Protons so I'm a little familiar with that. It's not my area
so I only know a little bit about the NGS setup.
The rapid-run mode of the HiSeq 2500 is perfectly capable of producing 150 million 150bp paired-end reads. This equates to 45Gb of sequence data.
If we are sequencing 5Mb genomes, at 40X, we need 200Mb of sequence. 96 of those will therefore need ~20Gb of sequence, so as you see, a single lane of HiSeq 2500 easily copes.
ARK-Genomics runs a non-profit full cost recovery business model, which means we charge for reagents, staff time and equipment. So for that lane of sequencing, we would probably charge in the region of £2500.
We need to factor in the cost of libraries. In reality, we could make this cheaper via automation, but for the sake of ease, let’s say the library prep is £100 per sample. That’s £9600 on library prep.
That’s a total of £12100, or £126 per genome.
In reality, I think we could get library prep down to £50 per sample, This would bring the cost down to £76 per genome.
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...