Template matching issue

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Laina Nicole Hall

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Oct 20, 2025, 1:44:34 PMOct 20
to Warp
Hi Warp team,

I have been running template matching on some yeast tomograms and am not getting any picks above lowest threshold (3). Most picks have scores of rlnAutopickFigureOfMerit ~1.5.

I have tried:
  • different templates
  • different pixel size (10, 20 A)
  • whitening or no whitening
  • low pass filtering 

Any suggestions? Could this be an error somewhere in tomogram reconstruction?

image.png

Thanks in advance,
Laina

Alister Burt

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Oct 20, 2025, 3:42:56 PMOct 20
to Laina Nicole Hall, Warp
Hey Laina,

Your image hasn’t come through so hard to tell what’s going on but a few questions I would ask myself
- are my tilt series aligned well (are features in tomograms “crispy”) 
- is my orientation sampling high enough?
- is my template good? 

I would also look directly at the cc volumes to see whether it is a problem with the matching process or just the peak picking on top of that :-) 

Good luck!

Alister


Sent from mobile - apologies for brevity

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