Template matching issue

[Laina Nicole Hall]

Hi Warp team,

I have been running template matching on some yeast tomograms and am not getting any picks above lowest threshold (3). Most picks have scores of rlnAutopickFigureOfMerit ~1.5.

I have tried:

• different templates
• different pixel size (10, 20 A)
• whitening or no whitening
• low pass filtering 

Any suggestions? Could this be an error somewhere in tomogram reconstruction?

Thanks in advance,

Laina

[Alister Burt]

Hey Laina,

Your image hasn’t come through so hard to tell what’s going on but a few questions I would ask myself

- are my tilt series aligned well (are features in tomograms “crispy”) 

- is my orientation sampling high enough?

- is my template good? 

I would also look directly at the cc volumes to see whether it is a problem with the matching process or just the peak picking on top of that :-) 

Good luck!

Alister

Sent from mobile - apologies for brevity

On Oct 20, 2025, at 11:44, 'Laina Nicole Hall' via Warp <war...@googlegroups.com> wrote:

Hi Warp team,

--
You received this message because you are subscribed to the Google Groups "Warp" group.
To unsubscribe from this group and stop receiving emails from it, send an email to warp-em+u...@googlegroups.com.
To view this discussion visit https://groups.google.com/d/msgid/warp-em/61763f02-0322-4c5f-b20b-ff77e6ad1d57n%40googlegroups.com.