I don't understand the question (what do you mean "use it" without "using ':'", etc.?), but:
The typical practice for autocommands is to put them in your vimrc (~/.vim/vimrc, etc.) or a local plugin (~/.vim/plugin/<name>.vim) so they are established during startup.
Some customizations don't required autocommands, though: if you're customizing the behavior of a particular filetype, then use an ftplugin, indent, or syntax script. (Or your filetype may already have knobs to tweak; see `:help :syn-file-remarks`, `:help ftplugin-docs`, `:help local-additions`, etc. Completing with `:help ft-<filetype><Ctrl-d>` is usually good too.)