guoyi.run: One-step TNT script for maximum parsimony phylogenetic

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Guoyi Zhang

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May 28, 2025, 6:14:58 AM5/28/25
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Hello everyone

I am sharing a new TNT script, guoyi.run

This tool is incredibly useful for anyone working with morphological phylogenetic analysis even molecular phylogenetics. It aims to make maximum parsimony phylogenetic analyses more reproducible and has already been adopted in numerous studies in entomology and palaeontology.

It provides a one-step TNT pipeline that supports multiple weighting schemes (equal, implied, and extended implied), customizable K values, consensus tree type selection, and resampling methods such as Bremer, bootstrap, and jackknife. It also automatically generates svg files and output files compatible with WinClada and FigTree. The entire analysis runs with a single command and intelligently adjusts tree search strategies based on dataset size.

Huge thanks to Pablo Goloboff, the author of TNT, for his encouragement and valuable comments.

Best regards
Guoyi

Guoyi Zhang

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May 28, 2025, 8:43:04 AM5/28/25
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For anyone who wants to get a copy of the PDF, feel free to look at my researchgate and website

Best regards
Guoyi

Jonathan Liria

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May 28, 2025, 8:52:12 AM5/28/25
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Hi Gouyi

Thanks!!!

JL

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PhD. Jonathan Liria
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Serdar Sönmez

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May 29, 2025, 7:14:52 AM5/29/25
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Hello Guoyi,

Thank you very much for sharing the script and the paper

Best

Jonathan Liria <jonatha...@gmail.com>, 28 May 2025 Çar, 15:52 tarihinde şunu yazdı:


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Prof. Dr. Serdar SÖNMEZ
Adıyaman Üniversitesi Fen Edebiyat Fakültesi Biyoloji Bölümü
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Anderson da Silva Nascimento

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Apr 3, 2026, 6:07:14 PM (6 days ago) Apr 3
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Hello, Guoyi!
Thanks for the script
I try to run it and this appears:
Captura de tela 2026-04-03 183616.png

Should i do something else before?
Sorry for the doubt, I've gone through your paper, but I’m still having a bit of trouble wrapping my head around the programming part.

Best regards,

Zhang Guoyi

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Apr 3, 2026, 8:34:29 PM (6 days ago) Apr 3
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I made a tutorial for that


Best regards 
Guoyi

Guoyi Zhang
School of Biological, Earth and Environmental Sciences
University of New South Wales
Australian Museum Research Institute
Australian Museum

From: tnt-tree-analysis-u...@googlegroups.com <tnt-tree-analysis-u...@googlegroups.com> on behalf of Anderson da Silva Nascimento <anders...@gmail.com>
Sent: Saturday, April 4, 2026 8:38:55 AM
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Subject: Re: guoyi.run: One-step TNT script for maximum parsimony phylogenetic
 
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