<$vars lastEntry="""{{{ [<Content>split[<br />]!is[blank]last[]] }}}""">
<$list filter="[<Content>split[<br />]!is[blank]]" variable="entry">
<<lastEntry>> <$text text=<<entry>>/> <br>
<$set name=isLast filter="[<entry>match<lastEntry>]" value="true" emptyValue="false">
<<isLast>>
</$set>
</$list>
</$vars>
relevant portion of the code below
<$vars lastEntry="""{{{ [<Content>split[<br />]!is[blank]last[]] }}}""">
<$list filter="[<Content>split[<br />]!is[blank]]" variable="entry">
<<lastEntry>> <$text text=<<entry>>/> <br>
<$set name=isLast filter="[<entry>match<lastEntry>]" value="true" emptyValue="false">
<<isLast>>
</$set>
</$list>
</$vars>lastEntry is the last split of <Content> with <br />
Use triple or single quotes for filter= assignments as they are strings.
But when passing a filter to a normal parameter you need no quotes and the triple braces to indicate that the parameter should be interpreted as a filter and not a literal string