Hive started as split this year and became increasingly defensive as it got bigger - now, two deeps, three supers, filled with bees, very strong. Hive is not queenless or recently swarmed; it's packed with bees. Not honey/pollen bound. And no ants.
I did reread "How to Tame a Hot Hive," which was circulated on this site earlier this year, and I will be using it with a few tweaks to requeen, hopefully next week.
Everytime I do a split or a virgin queen mates at this site, the hives seem to get more defensive, that's why I was interested in testing the genetics. I am wondering if there may be drones coming from defensive hives in the area. But that may be a futile effort because as "How to Tame a Hot Hive" notes, mitochondrial testing only tests the maternal genetic lineage.
Thanks, Brian