Problem with SpectraST

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Punit Kadimi

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Dec 1, 2012, 6:35:48 AM12/1/12
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Der all,

i am new to TPP tools i was trying to make spectral library from the search results of X!Tandem and getting some error the erroe message is CREATE: Libraries to be imported have mixed formats. Exiting.
i checked many times my process of processing till peptide prophet all was well but when i go with spectraST it just allways displays this error.

Below is the command which i am using with spectraST to create library,

spectrast.exe -cN <libname> -cP <prob>  <.pepXML files>

i will be very thankfull if any one suggest me whats going wrong.

regards,

Puneet

Paul.Un...@onlinehome.de

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Jun 23, 2020, 12:03:48 PM6/23/20
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I know this is probably not relevant for you anymore, but maybe somebody will search it in the future like I did.
I had a similar problem like you. My command was:

spectrast -cN<outputfilename> -cP<minProb> -co! <File1> [ <File2> … <FileN> ] 

as stated when using the spectrast -c command and reading the help.
However this doesnt work and I got the same error message as you, because spectraST recognised the "[" and "]" as librarys with different extensions.
So in my case the solution was simply to remove the brackets.

spectrast -cN<outputfilename> -cP<minProb> -co! <File1> <File2> … <FileN>

Maybe in your case its something similar, like removing "<" and ">".
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