Query related to PTMprophet tool in TPP

[Mehar Un Nissa]

Dear Sir/Madam,

I have query related to PTMprophet. 

I performed comet search for an mzml file and put it for further analysis as under:

 

-Using TPP tools Analyse peptides Checked for iprophet proteinprophet PTMprophet

-Mass added for PTMs were:

o79.966 Da for Phosphorylation- STY

o14.016 Da for Methylation- KR

o42.011 Da for Acetylation- K

Query: The analysis is getting failed with a warning message and an error saying

“unrecoganized mod taken….

Error: cannot initialize for sequence: SPRI….” as in snapshot below.

Could you please help me solve this error. 

Thanks

[David Shteynberg]

The error message refers to the M[147] modification, which is oxidation of Methionine.  Please specify this modification and rerun the tool.

Thanks for using the TPP!

-David

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