not getting significant hits while analyzing .raw file

[reeju]

Hi all,

I have been trying to analyze .raw file generated on waters synapt of standard protein BSA digested with trypsin. When the raw file is analyzed using progenesis software and mascot it is showing albumin as major hit. While when the same file is analyzed in TPP comet search we are not getting any hits of albumin. Database used is bovine proteome database from uniprot. 

Kindly suggest to resolve this issue.

Thanks in advance

With regards

Reeju