Precursor Intensity after Comet search

[Will Comstock]

Hello,

After Comet searching mzXML files, appending the "Precursor Intensity" column in the pepXML viewer reveals that it contains no values. Is there a way to make Comet retrieve/retain precursor intensities for later use? Would running XPRESS in label-free mode essentially accomplish this? I am using TPP 6.0 for these analyses. 

Respectfully,

-Will Comstock

[David Shteynberg]

Hi Will,

You can use option -PREC in advanced options for xinteract "Analyze Peptides" results to populate this column.    Alternatively you can run InteractParser with -I flag to get the same values in the pepXML results.

Cheers,

-David

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[Will Comstock]

Thank you David, exactly what I was looking for!

Best,

-Will