TPP 6.0 Libra Error

[Will Comstock]

Hello,

Our lab just updated from 5.2 to 6.0 and is testing some datasets that were formerly searched with 5.2.

We are now consistently encountering an error when running Libra after PeptideProphet, preventing the reporter ion quantitation from completing. The resulting interact.pep.xml is still able to be opened, there is just no Libra result appended to the table. 

Here is the Libra error encountered:

The return code is 29696. What could be causing this, and how might we try fixing it?

Thank you,

-Will Comstock

[David Shteynberg]

Hello William,

Sorry to hear you are having trouble with Libra.  If you are able to post the data for me to reproduce the problem I will attempt to patch it up.

Thank you!

-David

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[Will Comstock]

Hi David,

Here is a Google Drive folder containing all the files used in the search. A .txt file with the error message I encountered is included, as well as a screenshot of the Peptide Prophet parameters I used. 

https://drive.google.com/drive/folders/1xS8GdvOFw7tVMNLzObC_3tz1cttX_kLw?usp=sharing

I appreciate the help!

-Will

[David Shteynberg]

Hi Will,

Thanks for sharing the dataset.  I was able to reproduce the problem.  When I have a potential solution I will let you know.  Thank you for helping by reporting the error, it helps us improve the quality of the TPP over time.

-David

To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/107618af-5a58-4508-84eb-23a6598121a9n%40googlegroups.com.

[David Shteynberg]

Hi Will, 

I was able to trace this to a memory issue in the code and correct it.   I have posted a patched version here:

https://drive.google.com/file/d/1jiGuyLDC1E504GvnG_MqX7R6J4NWe_2D/view?usp=sharing

Replace your copy in C:\TPP\bin or where you installed your TPP.

Please let me know if you have any questions or issues with the new executable.

Thanks,

-David

To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/107618af-5a58-4508-84eb-23a6598121a9n%40googlegroups.com.

[Will Comstock]

Hi David,

It works! Thanks very much for addressing this issue so promptly. 

Best,

-Will

[David Shteynberg]

Great thanks for finding the bug Will!  The code will be part of the next release.

To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/9d31769a-e7a1-469d-ace1-7428a0c97ef3n%40googlegroups.com.