modified a.a.

[大木JAIST]

Hi Woonghee,

 

I have a question how I can describe non-standard amino acid in the sequence file.

My peptide has PCA (pyroglutamate) at the N-terminal end.

When I submit the sequence file with 3-letter code, PONDEROSA ignores the first residue.

The same file can be read in CYANA2.1 installed in our local linux machine.

Could you give me how I should write the N-terminal residue?

 

Thank you for your help.

 

Regards,

 

OHKI, Shinya

[Lee, Woonghee]

Hi Shinya,

You should stick with standard sequence until you collect enough restraints to fold the structure first. Then, you can operate Xplor-NIH with them and you use non standard sequence at that point.

Best,

Woonghee

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Sent: Wednesday, July 28, 2021 8:50:03 PM
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Subject: modified a.a.

 

[External Email - Use Caution]Hi Woonghee,

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