Apparent Kd determination based on Intensity from solution NMR titration data

Arun Gupta

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Jul 21, 2024, 10:25:13 AM7/21/24

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Dear Woonghee,

I am quite interested in determining the per-residue and global, apparent equilibrium dissociation constant K_d values based on the changes in signal intensities as a function of titrant concentrations in solution NMR titrations for my protein-protein complex datasets. I was just wondering if it's possible to include within the Poky software package a specific functionality or a python script to do the apparent K_ddetermination based on cross-peak signal intensities from solution 15N-HSQC titration data for protein-protein complexes. I think this sort of analysis will be helpful to a lot of the Poky users. Please find a relevant article reference i.e Nat. Chem. 11, 2019, 913 for your consideration.

Thanks in advance,

Arun

--

Arun Gupta PhD, MRSC

Research Fellow

Challis & Lewandowski Group

Chemistry Department

University of Warwick
Gibbet Hill Road
Coventry CV4 7AL
United Kingdom

Lee, Woonghee

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Jul 21, 2024, 12:21:18 PM7/21/24

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Have you checked the POKY Notepad? File-> Poky Scripts-> Find Additional... then search kd. Let me know if that works for you.

--

Woonghee Lee, Ph.D.

Assistant Professor of Chemistry

University of Colorado Denver

woongh...@ucdenver.edu

https://poky.clas.ucdenver.edu

https://poky.clas.ucdenver.edu/wlee-group

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