Issue with pi command

[Tanveer Shaikh]

Hello Dr. Lee,

I am doing some quantitative linewidth analysis of my protein. I selected all the peaks (pa) and centered them (pc), then I integrated them using the command "pi". I observed that the peaks are no longer centered. I tried doing it manually, but still, the peaks are shifting and not centered. Is there any way that the peak stays centered?

Regards

Tanveer Shaikh

Mississippi State University

[Lee, Woonghee]

Hi,

 

Please use “it” peak integration option.

 

Best,

 

Woonghee Lee, I.E.I.P., M.S., Ph.D.

 

Assistant Professor & Data Science and Chemistry Certificate Program Director

Department of Chemistry

University of Colorado Denver

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woongh...@ucdenver.edu

 

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From: nmr-s...@googlegroups.com <nmr-s...@googlegroups.com> on behalf of Tanveer Shaikh <tanveers...@gmail.com>
Date: Monday, February 9, 2026 at 9:18 AM
To: NMR POKY/SPARKY USER GROUP <nmr-s...@googlegroups.com>
Subject: [NMR POKY/SPARKY] Issue with pi command

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