Fiber photometry/ bulk fluorescence

[HET]

Hi

We are thinking of trying a couple of fibre photometry experiments in several candidate mouse brain areas (far simpler, quicker surgeries, etc).

The miniscope (through OEPS/ LabMaker) seems to be at lower cost than a commercial fiber photometry system.

Can the miniscope be adapted to simply record bulk GCaMP fluorescence from a fiber?

Any comments?

Thanks!

[Richardson Leão]

https://www.nature.com/articles/s41598-019-39724-y

i remember seeing a company that manufacture that circuit but i could not find it

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Richardson N Leao, MD PhD,

Neurodynamics Lab, Head

Brain Institute, UFRN/Brazil

[Richardson Leão]

here, open ephys:

https://open-ephys.org/pycontrol/pyphotometry

I suggest you to explore the literature as there are major differences in the kind of data you can get from the 2 technologies

[Daniel Aharoni]

Hi HET,

This should be possible with the Miniscope by just focusing the Miniscope at the top of your fiber. That being said, often times photometry systems use a second wavelength to correct for motion of the brain and this is something the standard Miniscope cannot do as it only works for signal channel imaging. One possible solution to this is actually to fully image the brain region you want using a cranial window or relay GRIN lens and correct for motion of the brain using an image registration algorithm. If then all you want is photometry data you can sum or average all the pixels of the recording.