EMAN 2.91 final ( GITHUB: 2021-03-08 12:05 - commit: 81caed2 )
Your EMAN2 is running on: Mac OS 10.16 x86_64
Your Python version is: 3.7.9
I am working with a few negative stain data sets and am getting funky results for my particle outputs (see "bad" attachment). I have processed negative stain data in EMAN for quite some time, but haven't had this issue before. I processed this data in EMAN 2.22 (on a different computer) and get the correct results (see "good" attachment). This has happened one other negative stain data set as well, but I don't understand why, and most data sets turn out normal.
My work flow is pretty basic and use defaults: evaluate and import -> interactive particle picking -> manual ctf processing (auto fitting, structure factor, redo auto fitting, generate output) -> build sets. Looking at the CTF fits, they all look normal.
Any feedback would be great. Let me know if you need more information.
Adam Miller
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